PUBLICATION
Assessing miR-451 Activity and Its Role in Erythropoiesis
- Authors
- Kretov, D.A., Shafik, A.M., Cifuentes, D.
- ID
- ZDB-PUB-171017-12
- Date
- 2018
- Source
- Methods in molecular biology (Clifton, N.J.) 1680: 179-190 (Chapter)
- Registered Authors
- Cifuentes, Daniel
- Keywords
- Ago2, Erythrocyte, MicroRNA, Microinjection, Zebrafish, miR-451
- MeSH Terms
-
- Animals
- Argonaute Proteins/genetics
- Erythropoiesis/genetics*
- Gene Expression
- Gene Expression Regulation*
- Genes, Reporter
- MicroRNAs/genetics*
- Phenotype
- RNA Interference*
- RNA, Messenger/genetics
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 29030849 Full text @ Meth. Mol. Biol.
Citation
Kretov, D.A., Shafik, A.M., Cifuentes, D. (2018) Assessing miR-451 Activity and Its Role in Erythropoiesis. Methods in molecular biology (Clifton, N.J.). 1680:179-190.
Abstract
The ability to microinject small RNAs and mRNAs into zebrafish embryos, of different genetic backgrounds, allows for the precise dissection of microRNA processing pathways at the molecular level, while simultaneously provides insight into their physiologic role. Here, we apply such an approach to determine the impact of Argonaute 2 in the processing of miR-451, a vertebrate-specific microRNA required for terminal erythrocyte differentiation. This was achieved using fluorescent microRNA reporter sensor assays and phenotype rescue experiments.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping