PUBLICATION

Transmission of a common intestinal neoplasm in zebrafish by cohabitation

Authors
Burns, A.R., Watral, V., Sichel, S., Spagnoli, S., Banse, A.V., Mittge, E., Sharpton, T.J., Guillemin, K., Kent, M.L.
ID
ZDB-PUB-171013-5
Date
2017
Source
Journal of fish diseases   41(4): 569-579 (Journal)
Registered Authors
Guillemin, Karen, Kent, Michael, Mittge, Erika K.
Keywords
Mycoplasma, intestinal, neoplasia, transmission, zebrafish
MeSH Terms
  • Adenocarcinoma/microbiology
  • Animals
  • Carcinoma, Small Cell/microbiology
  • Fish Diseases/microbiology
  • Fish Diseases/transmission*
  • Intestinal Neoplasms*/microbiology
  • Mycoplasma Infections/microbiology
  • Mycoplasma Infections/transmission*
  • Mycoplasma penetrans/genetics
  • Mycoplasma penetrans/isolation & purification*
  • Mycoplasma penetrans/physiology*
  • RNA, Bacterial/genetics
  • RNA, Ribosomal, 16S/genetics
  • Zebrafish*
PubMed
29023774 Full text @ J. Fish Dis.
Abstract
Intestinal neoplasms are common in zebrafish (Danio rerio) research facilities. These tumours are most often seen in older fish and are classified as small cell carcinomas or adenocarcinomas. Affected fish populations always contain subpopulations with preneoplastic lesions, characterized by epithelial hyperplasia or inflammation. Previous observations indicated that these tumours are unlikely caused by diet, water quality or genetic background, suggesting an infectious aetiology. We performed five transmission experiments by exposure of naïve fish to affected donor fish by cohabitation or exposure to tank effluent water. Intestinal lesions were observed in recipient fish in all exposure groups, including transmissions from previous recipient fish, and moribund fish exhibited a higher prevalence of neoplasms. We found a single 16S rRNA sequence, most similar to Mycoplasma penetrans, to be highly enriched in the donors and exposed recipients compared to unexposed control fish. We further tracked the presence of the Mycoplasma sp. using a targeted PCR test on individual dissected intestines or faeces or tank faeces. Original donor and exposed fish populations were positive for Mycoplasma, while corresponding unexposed control fish were negative. This study indicates an infectious aetiology for these transmissible tumours of zebrafish and suggests a possible candidate agent of a Mycoplasma species.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes