PUBLICATION
Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues
- Authors
- Barros-Becker, F., Lam, P.Y., Fisher, R., Huttenlocher, A.
- ID
- ZDB-PUB-171004-4
- Date
- 2017
- Source
- Journal of Cell Science 130(22): 3801-3808 (Journal)
- Registered Authors
- Huttenlocher, Anna, Lam, Pui Ying
- Keywords
- Actin cytoskeleton, Macrophage, Microtubules, Migration, Neutrophil, Zebrafish
- MeSH Terms
-
- Animals
- Cell Movement
- Cell Polarity
- Cell Shape
- Cell Tracking
- Larva/cytology
- Macrophages/physiology*
- Microscopy, Fluorescence
- Microscopy, Video
- Microtubules/metabolism
- Neutrophils/physiology*
- Paxillin/physiology
- Peptide Hydrolases/physiology
- Zebrafish
- Zebrafish Proteins/physiology
- PubMed
- 28972134 Full text @ J. Cell Sci.
Citation
Barros-Becker, F., Lam, P.Y., Fisher, R., Huttenlocher, A. (2017) Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues. Journal of Cell Science. 130(22):3801-3808.
Abstract
Cell motility is required for diverse processes during immunity and inflammation. Classically, leukocyte motility is defined as an amoeboid type of migration, however some leukocytes, like macrophages, also employ a more mesenchymal mode of migration. Here, we sought to characterize the mechanisms that regulate neutrophil and macrophage migration in vivo by using real-time imaging of leukocyte motility within interstitial tissues in zebrafish larvae. Neutrophils displayed a rounded morphology and rapid protease-independent motility, lacked defined paxillin puncta, and had persistent rearward polarization of stable F-actin and the microtubule network. By contrast, macrophages displayed an elongated morphology with reduced speed and increased directional persistence and formed paxillin-containing puncta but had a less-defined polarization of the microtubule and actin networks. We also observed differential effects of protease inhibition, microtubule disruption and ROCK inhibition on the efficiency of neutrophil and macrophage motility. Taken together, our findings suggest that larval zebrafish neutrophils and macrophage display distinct modes of migration within interstitial tissues in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping