PUBLICATION

Fluorescent light exposure incites acute and prolonged immune responses in Zebrafish (Danio rerio) skin

Authors
Gonzalez, T.J., Lu, Y., Boswell, M., Boswell, W., Medrano, G., Walter, S., Ellis, S., Savage, M., Varga, Z.M., Lawrence, C., Sanders, G., Walter, R.B.
ID
ZDB-PUB-171003-8
Date
2017
Source
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   208: 87-95 (Journal)
Registered Authors
Lawrence, Christian, Varga, Zoltan M.
Keywords
Fluorescent light, RNA-Seq, Skin, Vertebrate, Zebrafish
MeSH Terms
  • Animals
  • Fish Proteins/genetics*
  • Fish Proteins/metabolism
  • Fluorescence
  • Gene Expression Profiling/methods
  • Gene Expression Regulation/radiation effects
  • Kinetics
  • Light*
  • Real-Time Polymerase Chain Reaction
  • Skin/immunology
  • Skin/metabolism
  • Skin/radiation effects*
  • Transcription, Genetic/drug effects
  • Transcriptome
  • Zebrafish*/genetics
  • Zebrafish*/immunology
  • Zebrafish*/metabolism
PubMed
28965927 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Abstract
Artificial light produces an emission spectrum that is considerably different than the solar spectrum. Artificial light has been shown to affect various behavior and physiological processes in vertebrates. However, there exists a paucity of data regarding the molecular genetic effects of artificial light exposure. Previous studies showed that one of the commonly used fluorescent light source (FL; 4100K or "cool white") can affect signaling pathways related to maintenance of circadian rhythm, cell cycle progression, chromosome segregation, and DNA repair/recombination in the skin of male Xiphophorus maculatus. These observations raise questions concerning the kinetics of the FL induced gene expression response, and which biological functions become modulated at various times after light exposure. To address these questions, we exposed zebrafish to 4100K FL and utilized RNASeq to assess gene expression changes in skin at various times (1 to 12h) after FL exposure. We found 4100K FL incites a robust early (1-2h) transcriptional response, followed by a more protracted late response (i.e., 4-12h). The early transcriptional response involves genes associated with cell migration/infiltration and cell proliferation as part of an overall increase in immune function and inflammation. The protracted late transcriptional response occurs within gene sets predicted to maintain and perpetuate the inflammatory response, as well as suppression of lipid, xenobiotic, and melatonin metabolism.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes