PUBLICATION
In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short communication
- Authors
- Sanjeewa, K.K.A., Fernando, I.P.S., Kim, S.Y., Kim, H.S., Ahn, G., Jee, Y., Jeon, Y.J.
- ID
- ZDB-PUB-171001-4
- Date
- 2017
- Source
- International journal of biological macromolecules 107(Pt A): 803-807 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Sargassum horneri, anti-inflammation, sulfated polysaccharide, zebrafish
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/administration & dosage
- Anti-Inflammatory Agents/chemistry*
- Dinoprostone/biosynthesis
- Fucose/administration & dosage
- Fucose/chemistry*
- Inflammation/chemically induced
- Inflammation/drug therapy*
- Lipopolysaccharides/toxicity
- Mice
- Molecular Weight
- Nitric Oxide/biosynthesis
- Polysaccharides/administration & dosage
- Polysaccharides/chemistry*
- Polysaccharides/isolation & purification
- RAW 264.7 Cells
- Sargassum/chemistry
- Signal Transduction/drug effects
- Sulfates/chemistry
- Zebrafish/embryology
- PubMed
- 28962845 Full text @ Int. J. Biol. Macromol.
Citation
Sanjeewa, K.K.A., Fernando, I.P.S., Kim, S.Y., Kim, H.S., Ahn, G., Jee, Y., Jeon, Y.J. (2017) In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short communication. International journal of biological macromolecules. 107(Pt A):803-807.
Abstract
Recent studies on crude and pure compounds from Sargassum horneri have shown promising bioactive properties. However, anti-inflammatory potentials of fucose-rich sulfated polysaccharides from S. horneri have not yet been discovered. In the present study, we evaluated the in vitro and in vivo anti-inflammatory activities of four polysaccharide fractions separated from membrane filters according to their molecular weights (<5kDa (f1), 5-10kDa (f2), 10-30kDa (f3), and >30kDa (f4)). According to the results, F4 fraction inhibited the lipopolysaccharides (LPS)-induced nitric oxide (NO) (IC50=87.12μg/mL) and prostaglandin E2 production as well as pro-inflammatory cytokine production in RAW 264.7 cells through down-regulating nuclear factor-κB signaling cascade. According to the results, f4 has a potential to down-regulate LPS-induced toxicity, cell death and NO production levels in LPS-induced in vivo zebrafish embryo model. These results suggest that f4 fraction has the potential to develop functional materials or drugs to treat inflammatory diseases.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping