ZFIN ID: ZDB-PUB-170929-2
Copper impairs zebrafish swimbladder development by down-regulating Wnt signaling
Xu, J., Zhang, R., Zhang, T., Zhao, G., Huang, Y., Wang, H., Liu, J.X.
Date: 2017
Source: Aquatic toxicology (Amsterdam, Netherlands)   192: 155-164 (Journal)
Registered Authors: Liu, Jing-xia, Zhang, Ting
Keywords: BIO, Cu(2+), CuNPs, Swimbladder, Wnt signaling
MeSH Terms:
  • Air Sacs/drug effects
  • Air Sacs/embryology*
  • Air Sacs/metabolism
  • Animals
  • Copper/toxicity*
  • Down-Regulation/drug effects*
  • Down-Regulation/genetics
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Female
  • Gene Expression Regulation, Developmental/drug effects
  • Indoles/pharmacology
  • Ions
  • Male
  • Models, Biological
  • Nanoparticles/toxicity
  • Organogenesis/drug effects*
  • Organogenesis/genetics
  • Oximes/pharmacology
  • Water Pollutants, Chemical/toxicity
  • Wnt Signaling Pathway/drug effects*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/physiology
  • Zebrafish Proteins/genetics
PubMed: 28957717 Full text @ Aquat. Toxicol.
Copper nanoparticles (CuNPs) are used widely in different fields due to their attractive and effective abilities in inhibiting bacteria and fungi, but little information is available about their biological effects and potential molecular mechanisms on fish development. Here, CuNPs and copper (II) ions (Cu2+) were revealed to inhibit the specification and formation of three layers of zebrafish embryonic posterior swimbladder and impair its inflation in a stage-specific manner. CuNPs and Cu2+ were also revealed to down-regulate Wnt signaling in embryos. Furthermore, Wnt agonist 6-Bromoindirubin-3'-oxime (BIO) was found to neutralize the inhibiting effects of CuNPs or Cu2+ or both on zebrafish swimbladder development. The integrated data here provide the first evidence that both CuNPs and Cu2+ act on the specification and growth of the three layers of swimbladder and inhibit its inflation by down-regulating Wnt signaling in a stage-specific manner during embryogenesis.