PUBLICATION
Production of live larvae following in vitro maturation of zebrafish oocytes
- Authors
- Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R., Streit, D.P.
- ID
- ZDB-PUB-170928-6
- Date
- 2018
- Source
- Theriogenology 105: 115-119 (Journal)
- Registered Authors
- Zhang, Tiantian
- Keywords
- Carp pituitary, Danio rerio, In vitro maturation, Ovarian follicles, Pituitary gonadotropins
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- In Vitro Oocyte Maturation Techniques/veterinary*
- Larva/physiology
- Oocytes/drug effects
- Oocytes/growth & development*
- Pituitary Gland/chemistry
- Tissue Extracts/administration & dosage
- Tissue Extracts/chemistry
- Tissue Extracts/pharmacology
- Zebrafish/physiology*
- PubMed
- 28950168 Full text @ Theriogenology
Citation
Silva, L.A., Marques, L.S., Zhang, T., Rodrigues, R.B., Eloy, L.R., Streit, D.P. (2018) Production of live larvae following in vitro maturation of zebrafish oocytes. Theriogenology. 105:115-119.
Abstract
This study aimed to assess the effects of carp pituitary extract (CPE), follicle stimulating hormone (FSH) and luteinizing hormone (LH) on zebrafish oocyte maturation and the ability of these mature oocytes to be fertilized and developed until hatching. Stage III follicles were matured in eight treatments: five concentrations of CPE (16, 32, 48, 64 and 80 μg/mL), one of FSH (0.5 μg/mL), one of LH (0.5 μg/mL), or one combination of FSH (0.5 μg/mL) and LH (0.5 μg/mL). Maturation rates in CPE treatments were 12.8% (16 μg/mL), 24.8% (32 μg/mL), 27.0% (48 μg/mL), 22.7% (64 μg/mL) and 9.6% (80 μg/mL); in FSH was 15.7% (0.5 μg/mL), in LH was 31.8% (0.5 μg/mL) and in FSH (0.5 μg/mL) combined with LH (0.5 μg/mL) it was 50.4%. In vitro fertilization was performed in all treatments; however, only the treatment combining FSH and LH resulted in fertilized oocytes. After maturation using FSH combined with LH, the cleavage rate was 33.3% and hatching rate of live larvae was 20.0%. These results showed that FSH combined with LH was effective in IVM of zebrafish oocyte.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping