ZFIN ID: ZDB-PUB-170912-4
Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish
Kim, D.H., Kim, J., Marques, J.C., Grama, A., Hildebrand, D.G.C., Gu, W., Li, J.M., Robson, D.N.
Date: 2017
Source: Nature Methods   14(11): 1107-1114 (Journal)
Registered Authors: Robson, Drew
Keywords: none
MeSH Terms:
  • Animals
  • Brain/physiology
  • Calcium/metabolism*
  • Microscopy/methods
  • Neurons/metabolism*
  • Swimming/physiology*
  • Zebrafish/physiology*
PubMed: 28892088 Full text @ Nat. Methods
Calcium imaging with cellular resolution typically requires an animal to be tethered under a microscope, which substantially restricts the range of behaviors that can be studied. To expand the behavioral repertoire amenable to imaging, we have developed a tracking microscope that enables whole-brain calcium imaging with cellular resolution in freely swimming larval zebrafish. This microscope uses infrared imaging to track a target animal in a behavior arena. On the basis of the predicted trajectory of the animal, we applied optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We combined this motion-cancellation system with differential illumination focal filtering, a variant of HiLo microscopy, which enabled us to image the brain of a freely swimming larval zebrafish for more than an hour. This work expands the repertoire of natural behaviors that can be studied with cellular-resolution calcium imaging to potentially include spatial navigation, social behavior, feeding and reward.