PUBLICATION
Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish
- Authors
- Kim, D.H., Kim, J., Marques, J.C., Grama, A., Hildebrand, D.G.C., Gu, W., Li, J.M., Robson, D.N.
- ID
- ZDB-PUB-170912-4
- Date
- 2017
- Source
- Nature Methods 14(11): 1107-1114 (Journal)
- Registered Authors
- Robson, Drew
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/physiology
- Calcium/metabolism*
- Microscopy/methods
- Neurons/metabolism*
- Swimming/physiology*
- Zebrafish/physiology*
- PubMed
- 28892088 Full text @ Nat. Methods
Citation
Kim, D.H., Kim, J., Marques, J.C., Grama, A., Hildebrand, D.G.C., Gu, W., Li, J.M., Robson, D.N. (2017) Pan-neuronal calcium imaging with cellular resolution in freely swimming zebrafish. Nature Methods. 14(11):1107-1114.
Abstract
Calcium imaging with cellular resolution typically requires an animal to be tethered under a microscope, which substantially restricts the range of behaviors that can be studied. To expand the behavioral repertoire amenable to imaging, we have developed a tracking microscope that enables whole-brain calcium imaging with cellular resolution in freely swimming larval zebrafish. This microscope uses infrared imaging to track a target animal in a behavior arena. On the basis of the predicted trajectory of the animal, we applied optimal control theory to a motorized stage system to cancel brain motion in three dimensions. We combined this motion-cancellation system with differential illumination focal filtering, a variant of HiLo microscopy, which enabled us to image the brain of a freely swimming larval zebrafish for more than an hour. This work expands the repertoire of natural behaviors that can be studied with cellular-resolution calcium imaging to potentially include spatial navigation, social behavior, feeding and reward.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping