PUBLICATION
Transposons As Tools for Functional Genomics in Vertebrate Models
- Authors
- Kawakami, K., Largaespada, D.A., Ivics, Z.
- ID
- ZDB-PUB-170911-5
- Date
- 2017
- Source
- Trends in genetics : TIG 33(11): 784-801 (Review)
- Registered Authors
- Ivics, Zoltan, Kawakami, Koichi
- Keywords
- animal models, forward genetics, genetic screens, genomics, insertional mutagenesis, stem cells
- MeSH Terms
-
- Animals
- DNA Transposable Elements*
- Genomics*
- Models, Genetic*
- Vertebrates/genetics*
- PubMed
- 28888423 Full text @ Trends Genet.
Citation
Kawakami, K., Largaespada, D.A., Ivics, Z. (2017) Transposons As Tools for Functional Genomics in Vertebrate Models. Trends in genetics : TIG. 33(11):784-801.
Abstract
Genetic tools and mutagenesis strategies based on transposable elements are currently under development with a vision to link primary DNA sequence information to gene functions in vertebrate models. By virtue of their inherent capacity to insert into DNA, transposons can be developed into powerful tools for chromosomal manipulations. Transposon-based forward mutagenesis screens have numerous advantages including high throughput, easy identification of mutated alleles, and providing insight into genetic networks and pathways based on phenotypes. For example, the Sleeping Beauty transposon has become highly instrumental to induce tumors in experimental animals in a tissue-specific manner with the aim of uncovering the genetic basis of diverse cancers. Here, we describe a battery of mutagenic cassettes that can be applied in conjunction with transposon vectors to mutagenize genes, and highlight versatile experimental strategies for the generation of engineered chromosomes for loss-of-function as well as gain-of-function mutagenesis for functional gene annotation in vertebrate models, including zebrafish, mice, and rats.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping