PUBLICATION

Transposons As Tools for Functional Genomics in Vertebrate Models

Authors
Kawakami, K., Largaespada, D.A., Ivics, Z.
ID
ZDB-PUB-170911-5
Date
2017
Source
Trends in genetics : TIG   33(11): 784-801 (Review)
Registered Authors
Ivics, Zoltan, Kawakami, Koichi
Keywords
animal models, forward genetics, genetic screens, genomics, insertional mutagenesis, stem cells
MeSH Terms
  • Animals
  • DNA Transposable Elements*
  • Genomics*
  • Models, Genetic*
  • Vertebrates/genetics*
PubMed
28888423 Full text @ Trends Genet.
Abstract
Genetic tools and mutagenesis strategies based on transposable elements are currently under development with a vision to link primary DNA sequence information to gene functions in vertebrate models. By virtue of their inherent capacity to insert into DNA, transposons can be developed into powerful tools for chromosomal manipulations. Transposon-based forward mutagenesis screens have numerous advantages including high throughput, easy identification of mutated alleles, and providing insight into genetic networks and pathways based on phenotypes. For example, the Sleeping Beauty transposon has become highly instrumental to induce tumors in experimental animals in a tissue-specific manner with the aim of uncovering the genetic basis of diverse cancers. Here, we describe a battery of mutagenic cassettes that can be applied in conjunction with transposon vectors to mutagenize genes, and highlight versatile experimental strategies for the generation of engineered chromosomes for loss-of-function as well as gain-of-function mutagenesis for functional gene annotation in vertebrate models, including zebrafish, mice, and rats.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes