PUBLICATION
The in vitro zebrafish heart as a model to investigate the chronotropic effects of vapor anesthetics
- Authors
- Stoyek, M.R., Schmidt, M.K., Wilfart, F.M., Croll, R.P., Smith, F.M.
- ID
- ZDB-PUB-170908-12
- Date
- 2017
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 313(6): R669-R679 (Journal)
- Registered Authors
- Croll, Roger P.
- Keywords
- autonomic nervous system, desflurane, intracardiac nervous system, isoflurane, sevoflurane
- MeSH Terms
-
- Anesthetics, Inhalation/toxicity*
- Animals
- Biological Clocks/drug effects
- Bradycardia/chemically induced*
- Bradycardia/physiopathology
- Dose-Response Relationship, Drug
- Electric Stimulation
- Female
- Gases
- Heart/drug effects*
- Heart/innervation
- Heart/physiopathology
- Heart Rate/drug effects*
- Isoflurane/analogs & derivatives*
- Isoflurane/toxicity*
- Isolated Heart Preparation
- Male
- Methyl Ethers/toxicity*
- Models, Animal
- Sympathetic Nervous System/drug effects
- Sympathetic Nervous System/physiopathology
- Time Factors
- Vagus Nerve/drug effects
- Vagus Nerve/physiopathology
- Zebrafish*
- PubMed
- 28877873 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Stoyek, M.R., Schmidt, M.K., Wilfart, F.M., Croll, R.P., Smith, F.M. (2017) The in vitro zebrafish heart as a model to investigate the chronotropic effects of vapor anesthetics. American journal of physiology. Regulatory, integrative and comparative physiology. 313(6):R669-R679.
Abstract
In addition to their intended clinical actions, all general anesthetic agents in common use have detrimental intra- and post-surgical side effects on organs and systems including the heart. The major cardiac side effect of anesthesia is bradycardia, which increases the probability of insufficient systemic perfusion during surgery. These side effects also occur in all vertebrate species so far examined, but the underlying mechanisms are not clear. The zebrafish heart is a powerful model for studying cardiac electrophysiology, employing the same pacemaker system and neural control as do mammalian hearts. In this study isolated zebrafish hearts were significantly bradycardic during exposure to the vapor anesthetics sevoflurane (SEVO), desflurane (DES) and isoflurane (ISO). Bradycardia induced by DES and ISO continued during pharmacological blockade of the intracardiac portion of the autonomic nervous system, but the chronotropic effect of SEVO was eliminated during blockade. Bradycardia evoked by vagosympathetic nerve stimulation was augmented during DES and ISO exposure; nerve stimulation during SEVO exposure had no effect. Together these results support the hypothesis that the cardiac chronotropic effect of SEVO occurs via a neurally mediated mechanism while DES and ISO act directly upon cardiac pacemaker cells via an as yet unknown mechanism.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping