PUBLICATION
Cerebrospinal fluid injection into adult zebrafish for disease research
- Authors
- Matsui, H., Matsui, N.
- ID
- ZDB-PUB-170903-1
- Date
- 2017
- Source
- Journal of neural transmission (Vienna, Austria : 1996) 124(12): 1627-1633 (Journal)
- Registered Authors
- Matsui, Hideaki
- Keywords
- Cerebrospinal fluid, Parkinson’s disease, Zebrafish, α-synuclein
- MeSH Terms
-
- Ammonium Chloride/administration & dosage
- Animals
- Animals, Genetically Modified
- CASP8 and FADD-Like Apoptosis Regulating Protein/genetics
- CASP8 and FADD-Like Apoptosis Regulating Protein/metabolism
- Cerebrospinal Fluid/drug effects*
- Embryo, Mammalian
- Injections, Intraventricular/instrumentation
- Injections, Intraventricular/methods*
- Lysosomes/drug effects
- Mice
- Microtubule-Associated Proteins/metabolism
- Zebrafish
- Zebrafish Proteins/metabolism
- alpha-Synuclein/administration & dosage
- PubMed
- 28865043 Full text @ J. Neural Transm.
Citation
Matsui, H., Matsui, N. (2017) Cerebrospinal fluid injection into adult zebrafish for disease research. Journal of neural transmission (Vienna, Austria : 1996). 124(12):1627-1633.
Abstract
A modified method of cerebrospinal fluid injection was developed for the efficient and reliable administration of substances to the zebrafish central nervous system. The accuracy of this modified method was evaluated using Alexa Fluor dye injection. A high survival ratio was achieved due to the simplicity of the procedure and ice-tricaine combined anaesthesia. To validate this new method, we injected ammonium chloride, which successfully blocked lysosome function resulting in elevated LC3-II and the accumulation of ubiquitinated proteins. Injection of human α-synuclein fibrils initiated a prion-like propagation of α-synuclein pathology in zebrafish. This method can be used to investigate the effects of various substances and the propagation of α-synuclein in the central nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping