PUBLICATION
Loss of Apela Peptide in Mice Causes Low Penetrance Embryonic Lethality and Defects in Early Mesodermal Derivatives
- Authors
- Freyer, L., Hsu, C.W., Nowotschin, S., Pauli, A., Ishida, J., Kuba, K., Fukamizu, A., Schier, A.F., Hoodless, P.A., Dickinson, M.E., Hadjantonakis, A.K.
- ID
- ZDB-PUB-170831-1
- Date
- 2017
- Source
- Cell Reports 20: 2116-2130 (Journal)
- Registered Authors
- Pauli, Andrea, Schier, Alexander
- Keywords
- APJ, Apela, Apelin, Aplnr, Elabela, Ende, Toddler, cardiovascular development, gastrulation, macrophages, micro-computed tomography, microCT, vascular remodeling
- MeSH Terms
-
- Survival Analysis
- Myocardium/pathology
- Penetrance*
- Signal Transduction
- Embryo, Mammalian/abnormalities
- Embryo, Mammalian/pathology
- Embryonic Development
- Peptides/chemistry
- Peptides/metabolism*
- Gene Expression Regulation, Developmental
- CD11b Antigen/metabolism
- Embryo Loss/genetics*
- Embryo Loss/pathology*
- Mesoderm/embryology*
- Mesoderm/metabolism*
- Mice, Knockout
- Carrier Proteins/chemistry
- Carrier Proteins/metabolism*
- Phenotype
- Vascular Remodeling
- Amino Acid Sequence
- Mutation/genetics
- Endothelial Cells/metabolism
- Apelin Receptors/metabolism
- Myeloid Cells/metabolism
- Apelin/metabolism
- Up-Regulation/genetics
- Erythroid Cells/metabolism
- Animals
- PubMed
- 28854362 Full text @ Cell Rep.
Citation
Freyer, L., Hsu, C.W., Nowotschin, S., Pauli, A., Ishida, J., Kuba, K., Fukamizu, A., Schier, A.F., Hoodless, P.A., Dickinson, M.E., Hadjantonakis, A.K. (2017) Loss of Apela Peptide in Mice Causes Low Penetrance Embryonic Lethality and Defects in Early Mesodermal Derivatives. Cell Reports. 20:2116-2130.
Abstract
Apela (also known as Elabela, Ende, and Toddler) is a small signaling peptide that activates the G-protein-coupled receptor Aplnr to stimulate cell migration during zebrafish gastrulation. Here, using CRISPR/Cas9 to generate a null, reporter-expressing allele, we study the role of Apela in the developing mouse embryo. We found that loss of Apela results in low-penetrance cardiovascular defects that manifest after the onset of circulation. Three-dimensional micro-computed tomography revealed a higher penetrance of vascular remodeling defects, from which some mutants recover, and identified extraembryonic anomalies as the earliest morphological distinction in Apela mutant embryos. Transcriptomics at late gastrulation identified aberrant upregulation of erythroid and myeloid markers in mutant embryos prior to the appearance of physical malformations. Double-mutant analyses showed that loss of Apela signaling impacts early Aplnr-expressing mesodermal populations independently of the alternative ligand Apelin, leading to lethal cardiac defects in some Apela null embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping