|ZFIN ID: ZDB-PUB-170827-2|
Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle
Sztal, T.E., Currie, P.D., Bryson-Richardson, R.J.
|Source:||Methods in molecular biology (Clifton, N.J.) 1668: 27-35 (Chapter)|
|Registered Authors:||Bryson-Richardson, Robert, Currie, Peter D., Sztal, Tamar Esther|
|Keywords:||Pathology, RNA in situ hybridization, Skeletal muscle, Vibratome sectioning, Zebrafish adult skeletal muscle|
|PubMed:||28842900 Full text @ Meth. Mol. Biol.|
Sztal, T.E., Currie, P.D., Bryson-Richardson, R.J. (2017) Analysis of RNA Expression in Adult Zebrafish Skeletal Muscle. Methods in molecular biology (Clifton, N.J.). 1668:27-35.
ABSTRACTThe zebrafish is an excellent vertebrate model system to investigate skeletal muscle development and disease. During early muscle formation the small size of the developing zebrafish allows for the characterization of gene expression in whole embryos. However, as the zebrafish develops, access to the underlying skeletal muscle is limited, requiring the skeletal muscle to be sectioned for a more detailed examination. Here, we describe a straightforward and effective method to prepare adult zebrafish skeletal muscle sections, preserving muscle morphology, to characterize gene expression in the zebrafish adult skeletal muscle.
ADDITIONAL INFORMATION No data available