ZFIN ID: ZDB-PUB-170824-4
Claudin7b is required for the formation and function of inner ear in zebrafish
Li, X., Song, G., Zhao, Y., Zhao, F., Liu, C., Liu, D., Li, Q., Cui, Z.
Date: 2017
Source: Journal of Cellular Physiology   233(4): 3195-3206 (Journal)
Registered Authors: Cui, Zongbin, Li, Qing, Liu, Chunyan, Li, Xiaohui, Song, Guili, Zhao, Feng
Keywords: Claudin7b, hearing loss, inner ear, tight junction, zebrafish
MeSH Terms:
  • Animals
  • Ear, Inner/abnormalities
  • Ear, Inner/embryology*
  • Ear, Inner/metabolism*
  • Epithelial Cells/metabolism
  • Epithelium/metabolism
  • Epithelium/pathology
  • Gene Expression Regulation, Developmental
  • Hair Cells, Auditory, Inner/metabolism
  • Hair Cells, Auditory, Inner/pathology
  • Homozygote
  • Mutation/genetics
  • Tight Junctions/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 28834538 Full text @ J. Cell. Physiol.
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ABSTRACT
Zebrafish has become an excellent model for studying the development and function of inner ear. We report here a zebrafish line in which claudin 7b (cldn7b) locus is interrupted by a Tol2 transposon at its first intron. The homozygous mutants have enlarged otocysts, smaller or no otoliths, slowly formed semicircular canals, and insensitiveness to sound stimulation. These abnormal phenotypes and hearing loss of inner ear could be mostly rescued by injection of cldn7b-mRNA into one-cell stage homozygous mutant embryos. Mechanistically, cldn7b-deficiency interrupted the formation of apical junction complexes (AJCs) in otic epithelial cells of inner ear and the ion-homeostasis of endolymph, which then led to the loss of proper contact between otoliths and normally developed hair cells in utricle and saccule or aberrant mechanosensory transduction. Thus, Cldn7b is essential for the formation and proper function of inner ear through its unique role in keeping an initial integrity of otic epithelia during zebrafish embryogenesis. This article is protected by copyright. All rights reserved.
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