PUBLICATION

SERCA directs cell migration and branching across species and germ layers

Authors
Bower, D.V., Lansdale, N., Navarro, S., Truong, T.V., Bower, D.J., Featherstone, N.C., Connell, M.G., Al-Alam, D., Frey, M.R., Trinh, L.A., Fernandez, G.E., Warburton, D., Fraser, S.E., Bennett, D., Jesudason, E.C.
ID
ZDB-PUB-170820-2
Date
2017
Source
Biology Open   6(10): 1458-1471 (Journal)
Registered Authors
Fraser, Scott E.
Keywords
Branching morphogenesis, Calcium dynamics, Cell migration, SERCA
MeSH Terms
none
PubMed
28821490 Full text @ Biol. Open
Abstract
Branching morphogenesis underlies organogenesis in vertebrates and invertebrates, yet is incompletely understood. Here, we show that the sarco-endoplasmic reticulum Ca2+ reuptake pump (SERCA) directs budding across germ layers and species. Clonal knockdown demonstrated a cell-autonomous role for SERCA in Drosophila air sac budding. Live imaging of Drosophila tracheogenesis revealed elevated Ca2+ levels in migratory tip cells as they form branches. SERCA blockade abolished this Ca2+ differential, aborting both cell migration and new branching. Activating protein kinase C (PKC) rescued Ca2+ in tip cells and restored cell migration and branching. Likewise, inhibiting SERCA abolished mammalian epithelial budding, PKC activation rescued budding, while morphogens did not. Mesoderm (zebrafish angiogenesis) and ectoderm (Drosophila nervous system) behaved similarly, suggesting a conserved requirement for cell-autonomous Ca2+ signaling, established by SERCA, in iterative budding.
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