PUBLICATION

Live fate-mapping of joint-associated fibroblasts visualizes expansion of cell contributions during zebrafish fin regeneration

Authors
Tornini, V.A., Thompson, J.D., Allen, R.L., Poss, K.D.
ID
ZDB-PUB-170817-6
Date
2017
Source
Development (Cambridge, England)   144: 2889-2895 (Journal)
Registered Authors
Poss, Kenneth D., Thompson, John D., Tornini, Valerie A.
Keywords
Clonal analysis, Fin regeneration, Regeneration, Zebrafish
MeSH Terms
  • Animal Fins/cytology
  • Animal Fins/metabolism
  • Animals
  • Fibroblasts/cytology*
  • Fibroblasts/metabolism
  • Joints/metabolism
  • Joints/physiology
  • Regeneration/genetics
  • Regeneration/physiology*
  • Zebrafish
PubMed
28811310 Full text @ Development
Abstract
The blastema is a mass of progenitor cells responsible for regeneration of amputated salamander limbs and fish fins. Previous studies have indicated that resident cell sources producing the blastema contribute lineage-restricted progeny to regenerating tissue. However, these studies have labeled general cell types rather than granular cell subpopulations, and they do not explain the developmental transitions that must occur for distal structures to arise from cells with proximal identities in the appendage stump. Here, we find that regulatory sequences of tph1b, which encodes an enzyme that synthesizes serotonin, mark a subpopulation of fibroblast-like cells restricted to the joints of uninjured adult zebrafish fins. Amputation stimulates serotonin production in regenerating fin fibroblasts, yet targeted tph1b mutations abrogating this response do not disrupt fin regeneration. In uninjured animals, tph1b-expressing cells contribute fibroblast progeny that remain restricted to joints throughout life. By contrast, upon amputation, tph1b+ joint cells give rise to fibroblasts that distribute across the entire lengths of regenerating fin rays. Our experiments visualize and quantify how incorporation into an appendage blastema broadens the progeny contributions of a cellular subpopulation that normally has proximodistal restrictions.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping