PUBLICATION
Differential Behavioral Effects Of Ethanol Pre-exposure In Male And Female Zebrafish (Danio rerio)
- Authors
- Clayman, C.L., Malloy, E.J., Kearns, D.N., Connaughton, V.P.
- ID
- ZDB-PUB-170812-6
- Date
- 2017
- Source
- Behavioural brain research 335: 174-184 (Journal)
- Registered Authors
- Connaughton, Victoria P.
- Keywords
- Alcohol, Conditioned Place Preference, Juvenile, Novel Tank, Shoaling, Zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Conditioning, Classical/drug effects
- Conditioning, Psychological/drug effects
- Dose-Response Relationship, Drug
- Ethanol/pharmacology*
- Female
- Male
- Reward
- Sex Factors
- Zebrafish
- PubMed
- 28797598 Full text @ Behav. Brain Res.
Citation
Clayman, C.L., Malloy, E.J., Kearns, D.N., Connaughton, V.P. (2017) Differential Behavioral Effects Of Ethanol Pre-exposure In Male And Female Zebrafish (Danio rerio). Behavioural brain research. 335:174-184.
Abstract
Alcohol exposure in adolescence is a contributing factor toward reward-seeking behavior in adulthood. This reward-seeking behavior is assessed in animal models using the conditioned place preference (CPP) paradigm. In this study, ethanol-induced change in time spent by zebrafish on the initially non-preferred tank side was studied by conditioning adult zebrafish to ethanol dissolved in water (0.00% 1.00%; 1.25%; 1.50%; 1.60%; 1.75% vol/vol) paired with an initially non-preferred environment. Following a single conditioning cycle, fish swam unrestricted in the CPP chamber to assess changes in preference. Daily 20-minute pre-exposure to ethanol for 1 week during the juvenile stage starting at either 20days post fertilization (dpf) or 40 dpf altered percent time spent on the ethanol-paired side in adulthood in a dose-dependent and sex-dependent manner. The results suggest that male and female zebrafish are an effective model in which to investigate behavioral correlates of ethanol-induced changes in neural circuits implicated in reward and anxiety.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping