PUBLICATION
A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications
- Authors
- Fowler, D.K., Stewart, S., Seredick, S., Eisen, J.S., Stankunas, K., Washbourne, P.
- ID
- ZDB-PUB-170809-10
- Date
- 2016
- Source
- PLoS One 11: e0159277 (Journal)
- Registered Authors
- Eisen, Judith S., Stankunas, Kryn, Stewart, Scott, Washbourne, Philip
- Keywords
- Zebrafish, Expressed sequence tags, Recombination reactions, Vector cloning, Lentivirus, Neurons, Membrane proteins, Cloning
- MeSH Terms
-
- Animals
- Chromatography, Affinity/methods*
- Cloning, Molecular/methods*
- DNA, Recombinant/genetics*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Gene Expression*
- Genetic Vectors*
- Green Fluorescent Proteins/metabolism
- HEK293 Cells
- Hippocampus
- Humans
- RNA Interference*
- Rats
- Recombination, Genetic*
- Zebrafish/growth & development
- Zebrafish/metabolism
- PubMed
- 27500400 Full text @ PLoS One
Citation
Fowler, D.K., Stewart, S., Seredick, S., Eisen, J.S., Stankunas, K., Washbourne, P. (2016) A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. PLoS One. 11:e0159277.
Abstract
Recombination-based cloning is a quick and efficient way to generate expression vectors. Recent advancements have provided powerful recombinant DNA methods for molecular manipulations. Here, we describe a novel collection of three-fragment MultiSite Gateway cloning system-compatible vectors providing expanded molecular tools for vertebrate research. The components of this toolkit encompass a broad range of uses such as fluorescent imaging, dual gene expression, RNA interference, tandem affinity purification, chemically-inducible dimerization and lentiviral production. We demonstrate examples highlighting the utility of this toolkit for producing multi-component vertebrate expression vectors with diverse primary research applications. The vectors presented here are compatible with other Gateway toolkits and collections, facilitating the rapid generation of a broad range of innovative DNA constructs for biological research.
Errata / Notes
This article is corrected by ZDB-PUB-220906-70.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping