ZFIN ID: ZDB-PUB-170726-22
A Zebrafish Model for Evaluating the Function of Human Leukemic Gene IDH1 and Its Mutation
Ma, A.C.H., Shi, X., He, B.L., Guo, Y., Leung, A.Y.H.
Date: 2017
Source: Methods in molecular biology (Clifton, N.J.)   1633: 193-218 (Chapter)
Registered Authors: Leung, Anskar
Keywords: Hematopoiesis, Mutation, Myeloid malignancy, Myelopoiesis, Transient expression, Zebrafish
MeSH Terms:
  • Animals
  • Disease Models, Animal*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism*
  • Hematopoiesis
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Isocitrate Dehydrogenase/genetics*
  • Leukemia, Myeloid, Acute/genetics*
  • Leukemia, Myeloid, Acute/metabolism
  • Mutagenesis, Site-Directed
  • Mutation*
  • Phenotype
  • Zebrafish
PubMed: 28735489 Full text @ Meth. Mol. Biol.
ABSTRACT
The recent advent of next-generation sequencing (NGS) has greatly accelerated identification of gene mutations in myeloid malignancies at unprecedented speed that will soon outpace their functional validation by conventional laboratory techniques and animal models. A high-throughput whole-organism model is useful for the functional validation of new mutations. We recently reported the use of zebrafish to evaluate the hematopoietic function of isocitrate dehydrogenase 1 (IDH1) and the effects of expressing human IDH1-R132H that is frequently identified in human acute myeloid leukemia (AML), in myelopoiesis, with a view to develop zebrafish as a model of AML. Here, we use IDH1 as an example to describe a comprehensive approach to evaluate hematopoietic gene function and the effects of mutations using zebrafish as a model.
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