PUBLICATION
A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses
- Authors
- Fernando, I.P.S., Sanjeewa, K.K.A., Samarakoon, K.W., Lee, W.W., Kim, H.S., Kang, N., Ranasinghe, P., Lee, H.S., Jeon, Y.J.
- ID
- ZDB-PUB-170712-17
- Date
- 2017
- Source
- International journal of biological macromolecules 104(Pt A): 1185-1193 (Journal)
- Registered Authors
- Jeon, You-Jin
- Keywords
- Anti-inflammatory activity, Brown algae, Chnoospora minima, Fucoidan
- MeSH Terms
-
- Animals
- Anti-Inflammatory Agents/chemistry
- Anti-Inflammatory Agents/isolation & purification*
- Anti-Inflammatory Agents/pharmacology*
- Cyclooxygenase 2/metabolism
- Dinoprostone/metabolism
- Inflammation/chemically induced
- Inflammation/drug therapy
- Inflammation/metabolism
- Interleukin-1beta/metabolism
- Lipopolysaccharides/pharmacology*
- Macrophages/drug effects
- Macrophages/metabolism
- Mice
- Monosaccharides/analysis
- NF-kappa B/metabolism
- Nitric Oxide/biosynthesis
- Nitric Oxide Synthase Type II/metabolism
- Phaeophyceae/chemistry*
- Polysaccharides/chemistry
- Polysaccharides/isolation & purification*
- Polysaccharides/pharmacology*
- RAW 264.7 Cells
- PubMed
- 28690171 Full text @ Int. J. Biol. Macromol.
Citation
Fernando, I.P.S., Sanjeewa, K.K.A., Samarakoon, K.W., Lee, W.W., Kim, H.S., Kang, N., Ranasinghe, P., Lee, H.S., Jeon, Y.J. (2017) A fucoidan fraction purified from Chnoospora minima; a potential inhibitor of LPS-induced inflammatory responses. International journal of biological macromolecules. 104(Pt A):1185-1193.
Abstract
Fucoidans are an interesting group of bioactive sulfated polysaccharides abundant in brown seaweeds. The current study highlights the enrichment and extraction of fucoidan from Chnoospora minima by means of enzyme-assistant extraction using Celluclast and evaluation of its anti-inflammatory potential through in vitro and in vivo studies. The purified C. minima fucoidan (F2,4) inhibited the nitrous oxide (NO) production (IC50=27.82±0.88μg/ml) and expression of PGE2 through the subsequent downregulation of iNOS and COX-2 expression in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophages. F2,4 downregulated TNF-α, IL1-β, and IL-6 in RAW 264.7 macrophages in a dose-dependent manner and suppressed NO and ROS production in LPS stimulated zebrafish embryos while exerting a protective effect against the cell damage caused by LPS. Polysaccharide structural characterization was performed using FTIR, HPAE-PAD analysis of the monosaccharide content and NMR spectroscopy. Current findings confirm the potential anti-inflammatory activity of fucoidan purified from C. minima and elaborate its potential application as a functional ingredient in consumer products.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping