PUBLICATION

A Simple and Inexpensive Microinjection System for Zebrafish Embryos and Larvae

Authors
Samaee, S.M., Nikkhah, H., Varga, Z.M., Rezaei, B.
ID
ZDB-PUB-170706-2
Date
2017
Source
Zebrafish   14(6): 581-585 (Journal)
Registered Authors
Varga, Zoltan M.
Keywords
embryo-larva, microinjection setup, zebrafish
MeSH Terms
  • Aniline Compounds/administration & dosage
  • Animals
  • Coloring Agents/administration & dosage
  • Embryo, Nonmammalian/drug effects
  • Larva/drug effects
  • Microinjections/methods
  • Microinjections/veterinary*
  • Zebrafish/embryology*
PubMed
28678656 Full text @ Zebrafish
Abstract
Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems. Injection success with this low-cost system was verified based on the presence of injected dyes in zebrafish embryos, the absence of any significant morphological and behavioral differences between 3,4,-dichloroaniline-treated and untreated embryos, and larval viability.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes