PUBLICATION
Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules
- Authors
- Dorsemans, A.C., Lefebvre d'Hellencourt, C., Ait-Arsa, I., Jestin, E., Meilhac, O., Diotel, N.
- ID
- ZDB-PUB-170704-3
- Date
- 2017
- Source
- Journal of visualized experiments : JoVE (124): (Journal)
- Registered Authors
- Diotel, Nicolas
- Keywords
- none
- MeSH Terms
-
- Acute Disease
- Animals
- Chronic Disease
- Disease Models, Animal
- Hyperglycemia/diagnosis*
- Male
- Neurogenesis/physiology*
- Positron Emission Tomography Computed Tomography/methods*
- Tissue Distribution
- Zebrafish
- Zebrafish Proteins/metabolism
- PubMed
- 28671660 Full text @ J. Vis. Exp.
Citation
Dorsemans, A.C., Lefebvre d'Hellencourt, C., Ait-Arsa, I., Jestin, E., Meilhac, O., Diotel, N. (2017) Acute and Chronic Models of Hyperglycemia in Zebrafish: A Method to Assess the Impact of Hyperglycemia on Neurogenesis and the Biodistribution of Radiolabeled Molecules. Journal of visualized experiments : JoVE. (124).
Abstract
Hyperglycemia is a major health issue that leads to cardiovascular and cerebral dysfunction. For instance, it is associated with increased neurological problems after stroke and is shown to impair neurogenic processes. Interestingly, the adult zebrafish has recently emerged as a relevant and useful model to mimic hyperglycemia/diabetes and to investigate constitutive and regenerative neurogenesis. This work provides methods to develop zebrafish models of hyperglycemia to explore the impact of hyperglycemia on brain cell proliferation under homeostatic and brain repair conditions. Acute hyperglycemia is established using the intraperitoneal injection of D-glucose (2.5 g/kg bodyweight) into adult zebrafish. Chronic hyperglycemia is induced by immersing adult zebrafish in D-glucose (111 mM) containing water for 14 days. Blood-glucose-level measurements are described for these different approaches. Methods to investigate the impact of hyperglycemia on constitutive and regenerative neurogenesis, by describing the mechanical injury of the telencephalon, dissecting the brain, paraffin embedding and sectioning with a microtome, and performing immunohistochemistry procedures, are demonstrated. Finally, the method of using zebrafish as a relevant model for studying the biodistribution of radiolabeled molecules (here,[18F]-FDG) using PET/CT is also described.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping