ZFIN ID: ZDB-PUB-170629-5
Genetic Code Expansion in Zebrafish Embryos and Its Application to Optical Control of Cell Signaling
Liu, J., Hemphill, J., Samanta, S., Tsang, M., Deiters, A.
Date: 2017
Source: Journal of the American Chemical Society   139(27): 9100-9103 (Journal)
Registered Authors: Tsang, Michael
Keywords: none
MeSH Terms:
  • Amino Acids/genetics*
  • Amino Acids/metabolism
  • Animals
  • Genetic Code
  • Light*
  • Luciferases/genetics*
  • Luciferases/metabolism
  • Phosphotransferases/genetics*
  • Phosphotransferases/metabolism
  • Signal Transduction*
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed: 28657738 Full text @ J. Am. Chem. Soc.
ABSTRACT
Site-specific incorporation of unnatural amino acids into proteins provides a powerful tool to study protein function. Here we report genetic code expansion in zebrafish embryos and its application to the optogenetic control of cell signaling. We genetically encoded four unnatural amino acids with a diverse set of functional groups, which included a photocaged lysine that was applied to the light-activation of luciferase and kinase activity. This approach enables versatile manipulation of protein function in live zebrafish embryos, a transparent and commonly used model organism to study embryonic development.
ADDITIONAL INFORMATION