PUBLICATION

Reciprocal analyses in zebrafish and medaka reveal that harnessing the immune response promotes cardiac regeneration

Authors
Stainier, D.Y., Lai, S.B., Marín-Juez, R., Moura, P.L., Kuenne, C., Lai, J.K.H., Tsedeke, A.T., Guenther, S., Looso, M.
ID
ZDB-PUB-170622-8
Date
2017
Source
eLIFE   6: (Journal)
Registered Authors
Lai, Shih-Lei (Ben), Marín-Juez, Rubén, Stainier, Didier
Keywords
developmental biology, stem cells, zebrafish
Datasets
GEO:GSE94617
MeSH Terms
  • Animals
  • Cell Proliferation
  • Gene Expression Profiling
  • Heart Injuries/pathology*
  • Immunity, Cellular*
  • Macrophages/immunology
  • Myocytes, Cardiac/physiology
  • Neutrophils/immunology
  • Oryzias
  • Regeneration*
  • Zebrafish
PubMed
28632131 Full text @ Elife
Abstract
Zebrafish display a distinct ability to regenerate their heart following injury. However, this ability is not shared by another teleost, the medaka. In order to identify cellular and molecular bases for this difference, we performed comparative transcriptomic analyses following cardiac cryoinjury. This comparison points to major differences in immune cell dynamics between these models. Upon closer examination, we observed delayed and reduced macrophage recruitment in medaka, along with delayed neutrophil clearance. To investigate the role of immune responses in cardiac regeneration, we delayed macrophage recruitment in zebrafish and observed compromised neovascularization, neutrophil clearance, cardiomyocyte proliferation and scar resolution. In contrast, stimulating Toll-like receptor signaling in medaka enhanced immune cell dynamics and promoted neovascularization, neutrophil clearance, cardiomyocyte proliferation and scar resolution. Altogether, these data provide further insight into the complex role of the immune response during regeneration, and serve as a platform to identify and test additional regulators of cardiac repair.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping