PUBLICATION
Dietary gossypol suppressed postprandial TOR signaling and elevated ER stress pathways in turbot (Scophthalmus maximus L.)
- Authors
- Bian, F., Jiang, H., Man, M., Mai, K., Zhou, H., Xu, W., He, G.
- ID
- ZDB-PUB-170622-17
- Date
- 2017
- Source
- American journal of physiology. Endocrinology and metabolism 312: E37-E47 (Journal)
- Registered Authors
- Xu, Wei
- Keywords
- ER stress, TOR, gossypol, turbot (Scophthalmus maximus L.), liver fibrosis
- MeSH Terms
-
- Animals
- Cell Line
- Chemokines/drug effects
- Cytokines/drug effects*
- Diet
- Endoplasmic Reticulum Stress/drug effects*
- Fibrosis
- Flatfishes
- Gossypol/pharmacology*
- In Vitro Techniques
- Liver/drug effects*
- Liver/pathology
- Muscle Fibers, Skeletal/drug effects
- Muscle Fibers, Skeletal/metabolism
- Postprandial Period
- RNA, Messenger/drug effects*
- RNA, Messenger/metabolism
- Real-Time Polymerase Chain Reaction
- Signal Transduction
- TOR Serine-Threonine Kinases/drug effects*
- TOR Serine-Threonine Kinases/metabolism
- Transcriptome/drug effects
- Zebrafish
- PubMed
- 27894064 Full text @ Am. J. Physiol. Endocrinol. Metab.
Citation
Bian, F., Jiang, H., Man, M., Mai, K., Zhou, H., Xu, W., He, G. (2017) Dietary gossypol suppressed postprandial TOR signaling and elevated ER stress pathways in turbot (Scophthalmus maximus L.). American journal of physiology. Endocrinology and metabolism. 312:E37-E47.
Abstract
Gossypol is known to be a polyphenolic compound toxic to animals. However, its molecular targets are far from fully characterized. To evaluate the physiological and molecular effects of gossypol, we chose turbot (Scophthalmus maximus L.), a carnivorous fish, as our model species. Juvenile turbots (7.83 ± 0.02 g) were fed diets containing gradient levels of gossypol at 0 (G0), 600 (G1), and 1,200 (G2) mg/kg diets for 11 wk. After the feeding trial, fish growth, body protein, and fat contents were significantly reduced in the G2 group compared with those of the G0 group (P < 0.05). Gossypol had little impact on digestive enzyme activities and intestine morphology. However, gossypol caused liver fibrosis and stimulated chemokine and proinflammatory cytokine secretions. More importantly, gossypol suppressed target of rapamycin (TOR) signaling and induced endoplasmic reticulum (ER) stress pathway in both the feeding experiment and cell cultures. Our results demonstrated that gossypol inhibited TOR signaling and elevated ER stress pathways both in vivo and in vitro, thus providing new mechanism of action of gossypol in nutritional physiology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping