ZFIN ID: ZDB-PUB-170621-2
Adaptive optics improves multiphoton super-resolution imaging
Zheng, W., Wu, Y., Winter, P., Fischer, R., Nogare, D.D., Hong, A., McCormick, C., Christensen, R., Dempsey, W.P., Arnold, D.B., Zimmerberg, J., Chitnis, A., Sellers, J., Waterman, C., Shroff, H.
Date: 2017
Source: Nature Methods   14(9): 869-872 (Journal)
Registered Authors: Chitnis, Ajay, Dempsey, William
Keywords: Optical imaging, Super-resolution microscopy
MeSH Terms:
  • Equipment Design
  • Equipment Failure Analysis
  • Feedback
  • Image Enhancement/instrumentation*
  • Image Enhancement/methods*
  • Lenses*
  • Microscopy, Fluorescence, Multiphoton/instrumentation*
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
PubMed: 28628128 Full text @ Nat. Methods
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.