PUBLICATION

Adaptive optics improves multiphoton super-resolution imaging

Authors
Zheng, W., Wu, Y., Winter, P., Fischer, R., Nogare, D.D., Hong, A., McCormick, C., Christensen, R., Dempsey, W.P., Arnold, D.B., Zimmerberg, J., Chitnis, A., Sellers, J., Waterman, C., Shroff, H.
ID
ZDB-PUB-170621-2
Date
2017
Source
Nature Methods   14(9): 869-872 (Journal)
Registered Authors
Chitnis, Ajay, Dempsey, William
Keywords
Optical imaging, Super-resolution microscopy
MeSH Terms
  • Equipment Design
  • Equipment Failure Analysis
  • Feedback
  • Image Enhancement/instrumentation*
  • Image Enhancement/methods*
  • Lenses*
  • Microscopy, Fluorescence, Multiphoton/instrumentation*
  • Microscopy, Fluorescence, Multiphoton/methods*
  • Reproducibility of Results
  • Sensitivity and Specificity
PubMed
28628128 Full text @ Nat. Methods
Abstract
We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ∼250 μm from the coverslip surface.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping