PUBLICATION
Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish
- Authors
- Ellis, J.L., Yin, C.
- ID
- ZDB-PUB-170602-6
- Date
- 2017
- Source
- Journal of visualized experiments : JoVE (123): (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Vessels/pathology
- Coloring Agents
- Eosine Yellowish-(YS)
- Ethanol/toxicity
- Fatty Liver, Alcoholic/pathology*
- Fatty Liver, Alcoholic/veterinary
- Fibrosis
- Hematoxylin
- Histological Techniques
- Larva
- Liver/blood supply
- Liver/drug effects
- Liver/pathology*
- Zebrafish
- PubMed
- 28570521 Full text @ J. Vis. Exp.
Citation
Ellis, J.L., Yin, C. (2017) Histological Analyses of Acute Alcoholic Liver Injury in Zebrafish. Journal of visualized experiments : JoVE. (123).
Abstract
Alcoholic Liver Disease (ALD) refers to damage to the liver due to acute or chronic alcohol abuse. It is among the leading causes of alcohol-related morbidity and mortality and affects more than 2 million people in the United States. A better understanding of the cellular and molecular mechanisms underlying alcohol-induced liver injury is crucial for developing effective treatment for ALD. Zebrafish larvae exhibit hepatic steatosis and fibrogenesis after just 24 h of exposure to 2% ethanol, making them useful for the study of acute alcoholic liver injury. This work describes the procedure for acute ethanol treatment in zebrafish larvae and shows that it causes steatosis and swelling of the hepatic blood vessels. A detailed protocol for Hematoxylin and Eosin (H&E) staining that is optimized for the histological analysis of the zebrafish larval liver, is also described. H&E staining has several unique advantages over immunofluorescence, as it marks all liver cells and extracellular components simultaneously and can readily detect hepatic injury, such as steatosis and fibrosis. Given the increasing usage of zebrafish in modeling toxin and virus-induced liver injury, as well as inherited liver diseases, this protocol serves as a reference for the histological analyses performed in all these studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping