PUBLICATION
Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue
- Authors
- Zhou, S.R., Guo, L., Wang, X., Liu, Y., Peng, W.Q., Liu, Y., Wei, X.B., Dou, X., Ding, M., Lei, Q.Y., Qian, S.W., Li, X., Tang, Q.Q.
- ID
- ZDB-PUB-170601-5
- Date
- 2017
- Source
- Molecular and cellular biology 37(16): e00058-17 (Journal)
- Registered Authors
- Wang, Xu
- Keywords
- WAT, cavin-1, acetylation, HSL, lipolysis
- MeSH Terms
-
- 3T3-L1 Cells
- Acetylation/drug effects
- Adipose Tissue, White/drug effects
- Adipose Tissue, White/metabolism*
- Amino Acid Sequence
- Animals
- Cell Membrane/drug effects
- Cell Membrane/metabolism
- HEK293 Cells
- Humans
- Lipase/metabolism
- Lipolysis*/drug effects
- Lysine/metabolism
- Male
- Membrane Proteins/chemistry
- Membrane Proteins/metabolism*
- Mice
- Mice, Inbred C57BL
- Niacinamide/pharmacology
- Protein Binding/drug effects
- RNA-Binding Proteins/chemistry
- RNA-Binding Proteins/metabolism*
- Sirtuin 1/metabolism
- Zebrafish
- p300-CBP Transcription Factors/metabolism
- PubMed
- 28559430 Full text @ Mol. Cell. Biol.
Citation
Zhou, S.R., Guo, L., Wang, X., Liu, Y., Peng, W.Q., Liu, Y., Wei, X.B., Dou, X., Ding, M., Lei, Q.Y., Qian, S.W., Li, X., Tang, Q.Q. (2017) Acetylation of Cavin-1 Promotes Lipolysis in White Adipose Tissue. Molecular and cellular biology. 37(16):e00058-17.
Abstract
White adipose tissue (WAT) serves as a reversible energy storage depot in the form of lipids in response to nutritional status. Cavin-1, an essential component in the biogenesis of caveolae, is a positive regulator of lipolysis in adipocytes. However, molecular mechanisms of cavin-1 in the modulation of lipolysis remain poorly understood. Here, we showed that cavin-1 was acetylated at lysines 291, 293, and 298 (3K), which was under nutritional regulation in WAT. We further identified GCN5 as the acetyltransferase and Sirt1 as the deacetylase of cavin-1. Acetylation-mimetic 3Q mutants of cavin-1 augmented fat mobilization in 3T3-L1 adipocytes and zebrafish. Mechanistically, acetylated cavin-1 preferentially interacted with hormone-sensitive lipase and recruited it to the caveolae, thereby promoting lipolysis. Our finding shed light on essential role of cavin-1 in regulating lipolysis via an acetylation-dependent manner in WAT.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping