PUBLICATION
DNA replication timing during development anticipates transcriptional programs and parallels enhancer activation
- Authors
- Siefert, J.C., Georgescu, C., Wren, J.D., Koren, A., Sansam, C.L.
- ID
- ZDB-PUB-170518-7
- Date
- 2017
- Source
- Genome research 27(8): 1406-1416 (Journal)
- Registered Authors
- Sansam, Chris, Siefert, Joseph
- Keywords
- none
- Datasets
- GEO:GSE85713
- MeSH Terms
-
- DNA Replication Timing*
- Animals
- Transcription, Genetic*
- High-Throughput Nucleotide Sequencing/methods
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Zebrafish/genetics*
- Zebrafish/growth & development*
- Genome
- Gene Expression Regulation, Developmental*
- Enhancer Elements, Genetic*
- PubMed
- 28512193 Full text @ Genome Res.
Citation
Siefert, J.C., Georgescu, C., Wren, J.D., Koren, A., Sansam, C.L. (2017) DNA replication timing during development anticipates transcriptional programs and parallels enhancer activation. Genome research. 27(8):1406-1416.
Abstract
In dividing cells, DNA replication occurs in a precise order, but many questions remain regarding the mechanisms of replication timing establishment and regulation. We now have generated genome-wide, high-resolution replication timing maps throughout zebrafish development. Unexpectedly, in the rapid cell cycles preceding the midblastula transition, a defined timing program was present that predicted the initial wave of zygotic transcription. Replication timing was thereafter progressively and continuously remodeled across the majority of the genome, and epigenetic changes involved in enhancer activation frequently paralleled developmental changes in replication timing. Strikingly, the long arm of chromosome 4 underwent a dramatic developmentally regulated switch to late replication during gastrulation, reminiscent of mammalian X chromosome inactivation. This study reveals that replication timing is dynamic and tightly linked to epigenetic and transcriptional changes throughout early zebrafish development. These data provide insight into the regulation and functions of replication timing and will enable further mechanistic studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping