PUBLICATION
The rise of photoresponsive protein technologies applications in vivo: a spotlight on zebrafish developmental and cell biology.
- Authors
- Chow, R.W., Vermot, J.
- ID
- ZDB-PUB-170418-6
- Date
- 2017
- Source
- F1000Research 6: (Review)
- Registered Authors
- Vermot, Julien
- Keywords
- CRE, embryogenesis, fluorescent proteins, optogenetic technology
- MeSH Terms
- none
- PubMed
- 28413613 Full text @ F1000Res
Citation
Chow, R.W., Vermot, J. (2017) The rise of photoresponsive protein technologies applications in vivo: a spotlight on zebrafish developmental and cell biology.. F1000Research. 6.
Abstract
The zebrafish ( Danio rerio) is a powerful vertebrate model to study cellular and developmental processes in vivo. The optical clarity and their amenability to genetic manipulation make zebrafish a model of choice when it comes to applying optical techniques involving genetically encoded photoresponsive protein technologies. In recent years, a number of fluorescent protein and optogenetic technologies have emerged that allow new ways to visualize, quantify, and perturb developmental dynamics. Here, we explain the principles of these new tools and describe some of their representative applications in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping