sept8a and sept8b mRNA expression in the developing and adult zebrafish
- Berger, C., Helmprobst, F., Chapouton, P., Lillesaar, C., Stigloher, C.
- Gene expression patterns : GEP 25-26: 8-21 (Journal)
- Registered Authors
- Chapouton, Prisca, Lillesaar, Christina, Stigloher, Christian
- Danio rerio, Neuronal development, RNA in situ hybridization, Septin, Zebrafish, sept8a, sept8b
- MeSH Terms
- Central Nervous System/chemistry
- Central Nervous System/growth & development*
- Gene Expression Regulation, Developmental
- Gills/growth & development
- In Situ Hybridization
- Rhombencephalon/growth & development
- Tissue Embedding
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics*
- 28414113 Full text @ Gene Expr. Patterns
Berger, C., Helmprobst, F., Chapouton, P., Lillesaar, C., Stigloher, C. (2017) sept8a and sept8b mRNA expression in the developing and adult zebrafish. Gene expression patterns : GEP. 25-26:8-21.
Septins are highly conserved GTP-binding proteins involved in numerous cellular processes. Despite a growing awareness of their roles in the cell biology, development and signal transmission in nervous systems, comparably little is known about precise septin expression. Here, we use the well-established model organism zebrafish (Danio rerio) to unravel the expression of sept8a and sept8b, with special focus on the CNS. We performed whole mount RNA in situ hybridization on zebrafish 1-4 dpf in combination with serial sectioning of epon-embedded samples as well as on brain sections of adult zebrafish to obtain precise histological mapping of gene expression. Our results show a common expression of both genes at embryonic stages, whereas sept8a is mainly restricted to the gill arches and sept8b to specific brain structures at later stages. Brains of adult zebrafish reveal a large spatial overlap of sept8a and sept8b expression with few regions uniquely expressing sept8a or sept8b. Our results indicate a neuronal expression of both genes, and additionally suggest expression of sept8b in glial cells. Altogether, this study provides a first detailed insight into the expression of sept8a and sept8b in zebrafish and contributes to a more comprehensive understanding of septin biology in vertebrate model systems.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes