PUBLICATION
The Transcription Factor Bach1 Suppresses the Developmental Angiogenesis of Zebrafish
- Authors
- Jiang, L., Yin, M., Xu, J., Jia, M., Sun, S., Wang, X., Zhang, J., Meng, D.
- ID
- ZDB-PUB-170411-7
- Date
- 2017
- Source
- Oxidative medicine and cellular longevity 2017: 2143875 (Journal)
- Registered Authors
- Wang, Xu
- Keywords
- none
- MeSH Terms
-
- Animals
- Basic-Leucine Zipper Transcription Factors/genetics
- Basic-Leucine Zipper Transcription Factors/metabolism*
- Cytoskeletal Proteins/metabolism
- Embryo, Nonmammalian
- Enzyme-Linked Immunosorbent Assay
- Gene Expression Regulation, Developmental/genetics
- Human Umbilical Vein Endothelial Cells/metabolism
- Humans
- Mice
- Microscopy, Confocal
- Neovascularization, Physiologic/drug effects
- Neovascularization, Physiologic/genetics*
- Real-Time Polymerase Chain Reaction
- Signal Transduction/genetics
- Vascular Endothelial Growth Factor A/genetics
- Vascular Endothelial Growth Factor A/metabolism
- Wnt Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 28392885 Full text @ Oxid Med Cell Longev
Citation
Jiang, L., Yin, M., Xu, J., Jia, M., Sun, S., Wang, X., Zhang, J., Meng, D. (2017) The Transcription Factor Bach1 Suppresses the Developmental Angiogenesis of Zebrafish. Oxidative medicine and cellular longevity. 2017:2143875.
Abstract
Bach1 disrupts Wnt/β-catenin signaling, reduces the proliferation, migration, and tube formation activity of endothelial cells (ECs), and suppresses angiogenesis in mice with surgically induced hind-limb ischemia (HLI). However, the function of Bach1 during developmental angiogenesis in zebrafish remains unclear. Here, we found that zebrafish Bach1 was expressed ubiquitously during early embryonic development in zebrafish. Bach1b mRNA injection of Tg(fli1:gfp) fish disrupted intersegmental vessels (ISV) and dorsal longitudinal anastomotic vessels (DLAV) and suppressed endogenous Wnt/β-catenin signaling and Wnt8a stimulated vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) gene expression at early embryonic stages of zebrafish. Furthermore, chromatin immunoprecipitation experiments confirmed that Bach1 occupied the TCF/LEF-binding site of the VEGF promoter in human umbilical vein endothelial cells (HUVECs). Bach1 inhibited VEGF transcription by recruiting histone deacetylase 1 (HDAC1) to the VEGF promoter in HUVECs. Exogenous administration of VEGF or IL-8 partially rescued Bach1-driven antiangiogenic functions in HUVECs. Taken together, these observations indicate that Bach1 suppresses the developmental angiogenesis of zebrafish and that this function is associated with declines in Wnt/β-catenin signaling and VEGF and IL-8 expression.
Errata / Notes
Corrigendum ZDB-PUB-181201-1
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping