ZFIN ID: ZDB-PUB-170330-6
MicroRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
Kasper, D.M., Moro, A., Ristori, E., Narayanan, A., Hill-Teran, G., Fleming, E., Moreno-Mateos, M., Vejnar, C.E., Zhang, J., Lee, D., Gu, M., Gerstein, M., Giraldez, A., Nicoli, S.
Date: 2017
Source: Developmental Cell   40: 552-565.e5 (Journal)
Registered Authors: Giraldez, Antonio, Kasper, Dionna, Moro, Albertomaria, Nicoli, Stefania, Ristori, Emma, Vejnar, Charles
Keywords: cardiovascular development, endothelial cells, environmental stress, miR-139, miR-223, miR-24, phenotypic variability, robustness, target gene networks, zebrafish mutants
Microarrays: GEO:GSE81335, GEO:GSE81340, GEO:GSE81341
MeSH Terms:
  • Animals
  • Arteries/embryology
  • Arteries/metabolism
  • Cell Count
  • Embryo, Nonmammalian/metabolism*
  • Endothelial Cells/metabolism
  • Gene Regulatory Networks
  • Genome
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism
  • Homozygote
  • MicroRNAs/genetics
  • MicroRNAs/metabolism*
  • Morphogenesis
  • Mutagenesis/genetics
  • Mutation/genetics
  • Phenotype
  • Pseudopodia/metabolism
  • Quantitative Trait, Heritable
  • Stress, Physiological
  • Vertebrates/embryology*
  • Vertebrates/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics
PubMed: 28350988 Full text @ Dev. Cell
Proper functioning of an organism requires cells and tissues to behave in uniform, well-organized ways. How this optimum of phenotypes is achieved during the development of vertebrates is unclear. Here, we carried out a multi-faceted and single-cell resolution screen of zebrafish embryonic blood vessels upon mutagenesis of single and multi-gene microRNA (miRNA) families. We found that embryos lacking particular miRNA-dependent signaling pathways develop a vascular trait similar to wild-type, but with a profound increase in phenotypic heterogeneity. Aberrant trait variance in miRNA mutant embryos uniquely sensitizes their vascular system to environmental perturbations. We discovered a previously unrecognized role for specific vertebrate miRNAs to protect tissue development against phenotypic variability. This discovery marks an important advance in our comprehension of how miRNAs function in the development of higher organisms.