PUBLICATION
Compatibility Study of Danggui Buxue Tang on Chemical Ingredients, Angiogenesis and Endothelial Function
- Authors
- Lin, P.L., Li, Z.C., Xie, R.F., Wang, Y.H., Zhou, X.
- ID
- ZDB-PUB-170323-7
- Date
- 2017
- Source
- Scientific Reports 7: 45111 (Journal)
- Registered Authors
- Wang, Youhua
- Keywords
- Cardiology, Plant sciences
- MeSH Terms
-
- Animals
- Chromatography, High Pressure Liquid
- Drug Stability
- Drugs, Chinese Herbal/chemistry*
- Drugs, Chinese Herbal/pharmacology*
- Endothelial Cells/drug effects*
- Endothelial Cells/metabolism*
- Medicine, Chinese Traditional
- Neovascularization, Physiologic/drug effects*
- Phytochemicals/chemistry*
- Rats
- Reproducibility of Results
- Sensitivity and Specificity
- Zebrafish
- PubMed
- 28327640 Full text @ Sci. Rep.
Citation
Lin, P.L., Li, Z.C., Xie, R.F., Wang, Y.H., Zhou, X. (2017) Compatibility Study of Danggui Buxue Tang on Chemical Ingredients, Angiogenesis and Endothelial Function. Scientific Reports. 7:45111.
Abstract
Danggui Buxue Tang (DBT) is a classic Chinese herbal formula which consists of Astragali mongholici Radix and Angelica sinensis Radix (ASR). For chemical ingredients, HPLC were performed. Results showed compared with single herbs, DBT decoction could promote the dissolution of ingredients such as ferulic acid and calycosin. Furthermore, when ratio of AMR to ASR was 5 to 1, synthetic score was the best. For angiogenesis, normal and injured zebrafish model were applied. Results showed DBT and its ingredients had angiogenesis effects on Sub Intestinal vessels (SIVs) of normal zebrafish. Meanwhile, DBT and its single herbs could also recover Inter-Segmental Vessels (ISVs) injured by VRI. Angiogenesis effects of DBT on ISVs were better than single herbs. AMR extract, Total Saponins of AMR, Polysaccharide of ASR, ferulic acid, calycosin and calycosin-7-glucoside could be effective ingredients for angiogenisis. For endothelium functions, Lysoph-Osphatidyl choline was used to damage rat endothelial function of thoracic aorta. The results showed DBT and its single herbs could improve endothelial dysfunctions in dose-dependence. Both ferulic acid and calycosin-7-glucoside could also improve endothelium dysfunction in dose dependence. Therefore, compatibility of DBT was reasonable. Compared with single herbs, DBT could promote dissolution of effective ingredients, enhance angiogenesis and relieve endothelial dysfunction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping