ZFIN ID: ZDB-PUB-170321-5
itFISH: Enhanced Staining by Iterative Fluorescent In Situ Hybridization
Row, R.H., Martin, B.L.
Date: 2017
Source: Zebrafish   14(6): 578-580 (Journal)
Registered Authors: Martin, Benjamin, Row, Richard H.
Keywords: fluorescent in situ hybridization, iterative, neuromesodermal progenitor
MeSH Terms:
  • Animals
  • Fluorescent Dyes/analysis*
  • In Situ Hybridization, Fluorescence/methods*
  • Staining and Labeling/methods*
  • Zebrafish/physiology*
PubMed: 28318417 Full text @ Zebrafish
Fluorescent in situ hybridization (FISH) is an important tool for zebrafish research, particularly when observing the expression of two different genes in the same embryo. Peroxidase-catalyzed deposition of tyramide-conjugated dyes is a widely used and cost-effective approach to performing FISH. A major limitation of the technique is that it does not work well for weakly expressed genes. Here we present a method adapted from planarian research for use in zebrafish that provides a dramatic enhancement of weak staining. By iterating the antibody staining and development steps, a strong signal can be obtained from probes that were previously too weak to detect.