PUBLICATION
Comparison of proteomic profiles in the zebrafish retina during experimental degeneration and regeneration
- Authors
- Eastlake, K., Heywood, W.E., Tracey-White, D., Aquino, E., Bliss, E., Vasta, G.R., Mills, K., Khaw, P.T., Moosajee, M., Limb, G.A.
- ID
- ZDB-PUB-170317-4
- Date
- 2017
- Source
- Scientific Reports 7: 44601 (Journal)
- Registered Authors
- Keywords
- Proteomics, Regeneration and repair in the nervous system
- MeSH Terms
-
- Animals
- Apolipoproteins/metabolism
- Cell Membrane/drug effects
- Cell Membrane/metabolism
- Cytoskeletal Proteins/metabolism
- Eye Proteins/metabolism
- Fibrin/metabolism
- Gene Ontology
- Histones/metabolism
- Injections
- Ouabain/administration & dosage
- Ouabain/pharmacology
- Proteomics/methods*
- Regeneration*/drug effects
- Reproducibility of Results
- Retina/drug effects
- Retina/metabolism*
- Retina/pathology
- Retinal Degeneration/metabolism*
- Retinal Degeneration/pathology
- Zebrafish/metabolism*
- Zebrafish Proteins/metabolism
- PubMed
- 28300160 Full text @ Sci. Rep.
Citation
Eastlake, K., Heywood, W.E., Tracey-White, D., Aquino, E., Bliss, E., Vasta, G.R., Mills, K., Khaw, P.T., Moosajee, M., Limb, G.A. (2017) Comparison of proteomic profiles in the zebrafish retina during experimental degeneration and regeneration. Scientific Reports. 7:44601.
Abstract
Zebrafish spontaneously regenerate the retina after injury. Although the gene expression profile has been extensively studied in this species during regeneration, this does not reflect protein function. To further understand the regenerative process in the zebrafish, we compared the proteomic profile of the retina during injury and upon regeneration. Using two-dimensional difference gel electrophoresis (2D-DIGE) and label-free quantitative proteomics (quadrupole time of flight LC-MS/MS), we analysed the retina of adult longfin wildtype zebrafish at 0, 3 and 18 days after Ouabain injection. Gene ontology analysis indicates reduced metabolic processing, and increase in fibrin clot formation, with significant upregulation of fibrinogen gamma polypeptide, apolipoproteins A-Ib and A-II, galectin-1, and vitellogenin-6 during degeneration when compared to normal retina. In addition, cytoskeleton and membrane transport proteins were considerably altered during regeneration, with the highest fold upregulation observed for tubulin beta 2 A, histone H2B and brain type fatty acid binding protein. Key proteins identified in this study may play an important role in the regeneration of the zebrafish retina and investigations on the potential regulation of these proteins may lead to the design of protocols to promote endogenous regeneration of the mammalian retina following retinal degenerative disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping