ZFIN ID: ZDB-PUB-170317-3
Zebrafish Caudal Haematopoietic Embryonic Stromal Tissue (CHEST) Cells Support Haematopoiesis
Wolf, A., Aggio, J., Campbell, C., Wright, F., Marquez, G., Traver, D., Stachura, D.L.
Date: 2017
Source: Scientific Reports   7: 44644 (Journal)
Registered Authors: Traver, David
Keywords: Stem-cell niche, Stem cells
MeSH Terms:
  • Animals
  • Cell Count
  • Cell Differentiation/drug effects
  • Cell Proliferation/drug effects
  • Cell Separation
  • Cell Shape/drug effects
  • Cells, Cultured
  • Embryo, Nonmammalian/cytology*
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Endothelial Cells/cytology
  • Endothelial Cells/drug effects
  • Endothelial Cells/metabolism
  • Fibroblast Growth Factor 2/pharmacology
  • Hematopoiesis*/drug effects
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/drug effects
  • Hematopoietic Stem Cells/metabolism
  • Selenium/pharmacology
  • Stromal Cells/cytology
  • Stromal Cells/drug effects
  • Stromal Cells/metabolism
  • Zebrafish/embryology*
PubMed: 28300168 Full text @ Sci. Rep.
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ABSTRACT
Haematopoiesis is an essential process in early vertebrate development that occurs in different distinct spatial locations in the embryo that shift over time. These different sites have distinct functions: in some anatomical locations specific hematopoietic stem and progenitor cells (HSPCs) are generated de novo. In others, HSPCs expand. HSPCs differentiate and renew in other locations, ensuring homeostatic maintenance. These niches primarily control haematopoiesis through a combination of cell-to-cell signalling and cytokine secretion that elicit unique biological effects in progenitors. To understand the molecular signals generated by these niches, we report the generation of caudal hematopoietic embryonic stromal tissue (CHEST) cells from 72-hours post fertilization (hpf) caudal hematopoietic tissue (CHT), the site of embryonic HSPC expansion in fish. CHEST cells are a primary cell line with perivascular endothelial properties that expand hematopoietic cells in vitro. Morphological and transcript analysis of these cultures indicates lymphoid, myeloid, and erythroid differentiation, indicating that CHEST cells are a useful tool for identifying molecular signals critical for HSPC proliferation and differentiation in the zebrafish. These findings permit comparison with other temporally and spatially distinct haematopoietic-supportive zebrafish niches, as well as with mammalian haematopoietic-supportive cells to further the understanding of the evolution of the vertebrate hematopoietic system.
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