ZFIN ID: ZDB-PUB-170316-3
Comparative analysis of monoaminergic cerebrospinal fluid-contacting cells in Osteichthyes (bony vertebrates)
Xavier, A.L., Fontaine, R., Bloch, S., Affaticati, P., Jenett, A., Demarque, M., Vernier, P., Yamamoto, K.
Date: 2017
Source: The Journal of comparative neurology   525(9): 2265-2283 (Journal)
Registered Authors: Demarque, Michael, Vernier, Philippe, Yamamoto, Kei
Keywords: CSF-contacting cells, PVO, RRID: AB_10000240, RRID: AB_2201528, RRID: AB_221448, RRID: AB_2314334, RRID: AB_2314655, RRID: AB_477522, RRID: AB_87181, RRID: AB_94865, dopamine, evolution, hypothalamus, monoamine, serotonin, ventricle
MeSH Terms:
  • Animals
  • Biogenic Monoamines/metabolism*
  • Biological Evolution
  • Brain/cytology*
  • Brain/metabolism
  • Cerebrospinal Fluid/physiology*
  • Chick Embryo
  • Chickens
  • ELAV Proteins/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Imaging, Three-Dimensional
  • Male
  • Neurons/classification
  • Neurons/metabolism*
  • RNA, Messenger/metabolism
  • Vertebrates
  • Xenopus
  • Zebrafish
  • Zonula Occludens-1 Protein/metabolism
PubMed: 28295297 Full text @ J. Comp. Neurol.
Cerebrospinal fluid-contacting (CSF-c) cells containing monoamines such as dopamine (DA) and serotonin (5-HT) occur in the periventricular zones of the hypothalamic region of most vertebrates except for placental mammals. Here we compare the organization of the CSF-c cells in chicken, Xenopus, and zebrafish, by analyzing the expression of synthetic enzymes of DA and 5-HT, respectively tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH), and draw an evolutionary scenario for this cell population. Due to the lack of TH immunoreactivity in this region, the hypothalamic CSF-c cells have been thought to take up DA from the ventricle instead of synthesizing it. We demonstrate that a second TH gene (TH2) is expressed in the CSF-c cells of all the three species, suggesting that these cells do indeed synthetize DA. Furthermore, we found that many CSF-c cells co-express TH2 and TPH1 and contain both DA and 5-HT, a dual neurotransmitter phenotype hitherto undescribed in the brain of any vertebrate. The similarities of CSF-c cells in chicken, Xenopus, and zebrafish suggest that these characteristics are inherited from the common ancestor of the Osteichthyes. A significant difference between tetrapods and teleosts is that teleosts possess an additional CSF-c cell population around the posterior recess (PR) that has emerged in specific groups of Actinopterygii. Our comparative analysis reveals that the hypothalamus in mammals and teleosts has evolved in a divergent manner: placental mammals have lost the monoaminergic CSF-c cells, while teleosts have increased their relative number.