PUBLICATION
Optical Projection Tomography as a Novel Method to Visualize and Quantitate Whole-Brain Patterns of Cell Proliferation in the Adult Zebrafish Brain
- Authors
- Lindsey, B.W., Kaslin, J.
- ID
- ZDB-PUB-170316-1
- Date
- 2017
- Source
- Zebrafish 14(6): 574-577 (Journal)
- Registered Authors
- Kaslin, Jan
- Keywords
- brain development, clearing, neurogenesis, optical projection tomography, regeneration, stem cell proliferation, three-dimensional imaging
- MeSH Terms
-
- Animals
- Brain/anatomy & histology*
- Brain/growth & development
- Cell Proliferation*
- Image Processing, Computer-Assisted/methods*
- Software
- Tomography, Optical/methods
- Tomography, Optical/veterinary*
- Zebrafish/anatomy & histology*
- Zebrafish/growth & development
- PubMed
- 28296621 Full text @ Zebrafish
Citation
Lindsey, B.W., Kaslin, J. (2017) Optical Projection Tomography as a Novel Method to Visualize and Quantitate Whole-Brain Patterns of Cell Proliferation in the Adult Zebrafish Brain. Zebrafish. 14(6):574-577.
Abstract
How distinct cell populations are distributed in three-dimensional space under homeostasis or following injury, neurodegeneration, or with senescence can teach us much about brain-wide patterns and signaling along the neuroaxis. Visualizing individual cell populations in the mature vertebrate central nervous system (CNS) has remained a challenge as a result of difficulty clearing adult brain tissue or limitations in imaging depth or resolution. We have developed a simple clearing and imaging pipeline optimally suited for the adult zebrafish brain to investigate changes in patterns of cell proliferation in wild-type and transgenic backgrounds that can easily be quantified and represented using FIJI and IMARIS software.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping