PUBLICATION
Neurotransmitter-Regulated Regeneration in the Zebrafish Retina
- Authors
- Rao, M.B., Didiano, D., Patton, J.G.
- ID
- ZDB-PUB-170314-11
- Date
- 2017
- Source
- Stem Cell Reports 8(4): 831-842 (Journal)
- Registered Authors
- Patton, James G.
- Keywords
- GABA, Müller glia, regeneration, retina, zebrafish
- MeSH Terms
-
- Animals
- Stem Cells/cytology
- Stem Cells/metabolism
- Signal Transduction
- Cell Proliferation*
- Regeneration*
- Retina/cytology
- Retina/physiology*
- Neuroglia/cytology
- Neuroglia/metabolism
- gamma-Aminobutyric Acid/metabolism*
- Zebrafish/physiology*
- Receptors, Glutamate/metabolism
- PubMed
- 28285877 Full text @ Stem Cell Reports
Citation
Rao, M.B., Didiano, D., Patton, J.G. (2017) Neurotransmitter-Regulated Regeneration in the Zebrafish Retina. Stem Cell Reports. 8(4):831-842.
Abstract
Current efforts to repair damaged or diseased mammalian retinas are inefficient and largely incapable of fully restoring vision. Conversely, the zebrafish retina is capable of spontaneous regeneration upon damage using Müller glia (MG)-derived progenitors. Understanding how zebrafish MG initiate regeneration may help develop new treatments that prompt mammalian retinas to regenerate. We show that inhibition of γ-aminobutyric acid (GABA) signaling facilitates initiation of MG proliferation. GABA levels decrease following damage, and MG are positioned to detect decreased ambient levels and undergo dedifferentiation. Using pharmacological and genetic approaches, we demonstrate that GABAA receptor inhibition stimulates regeneration in undamaged retinas while activation inhibits regeneration in damaged retinas.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping