PUBLICATION

Neurotransmitter-Regulated Regeneration in the Zebrafish Retina

Authors
Rao, M.B., Didiano, D., Patton, J.G.
ID
ZDB-PUB-170314-11
Date
2017
Source
Stem Cell Reports   8(4): 831-842 (Journal)
Registered Authors
Patton, James G.
Keywords
GABA, Müller glia, regeneration, retina, zebrafish
MeSH Terms
  • Zebrafish/physiology*
  • Receptors, Glutamate/metabolism
  • Animals
  • Signal Transduction
  • Cell Proliferation*
  • gamma-Aminobutyric Acid/metabolism*
  • Retina/cytology
  • Retina/physiology*
  • Regeneration*
  • Neuroglia/cytology
  • Neuroglia/metabolism
  • Stem Cells/cytology
  • Stem Cells/metabolism
(all 13)
PubMed
28285877 Full text @ Stem Cell Reports
Abstract
Current efforts to repair damaged or diseased mammalian retinas are inefficient and largely incapable of fully restoring vision. Conversely, the zebrafish retina is capable of spontaneous regeneration upon damage using Müller glia (MG)-derived progenitors. Understanding how zebrafish MG initiate regeneration may help develop new treatments that prompt mammalian retinas to regenerate. We show that inhibition of γ-aminobutyric acid (GABA) signaling facilitates initiation of MG proliferation. GABA levels decrease following damage, and MG are positioned to detect decreased ambient levels and undergo dedifferentiation. Using pharmacological and genetic approaches, we demonstrate that GABAA receptor inhibition stimulates regeneration in undamaged retinas while activation inhibits regeneration in damaged retinas.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
knu2TgTransgenic Insertion
    mi4TgTransgenic Insertion
      mi2001TgTransgenic Insertion
        nt19TgTransgenic Insertion
          pt303TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            ascl1aMO1-ascl1aMRPHLNO
            ascl1aMO2-ascl1aMRPHLNO
            1 - 2 of 2
            Show
            Fish
            No data available
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            RFPEFGRFP
            1 - 3 of 3
            Show
            Mapping
            No data available
            Your Input Welcome
            We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
            Citation
            Rao, M.B., Didiano, D., Patton, J.G. (2017) Neurotransmitter-Regulated Regeneration in the Zebrafish Retina. Stem Cell Reports. 8(4):831-842.