PUBLICATION
Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam
- Authors
- Xu, D., Zhou, W., Peng, L.
- ID
- ZDB-PUB-170309-5
- Date
- 2017
- Source
- Biomedical Optics Express 8: 570-578 (Journal)
- Registered Authors
- Zhou, Weibin
- Keywords
- Imaging systems, Fluorescence microscopy, Spectroscopy, fluorescence and luminescence, Three-dimensional microscopy
- MeSH Terms
- none
- PubMed
- 28270968 Full text @ Biomed. Opt. Express
Citation
Xu, D., Zhou, W., Peng, L. (2017) Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam. Biomedical Optics Express. 8:570-578.
Abstract
Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping