Exogenous gene integration mediated by genome editing technologies in zebrafish
- Morita, H., Taimatsu, K., Yanagi, K., Kawahara, A.
- Bioengineered 8(3): 287-295 (Review)
- Registered Authors
- Kawahara, Atsuo, Morita, Hitoshi
- CRISPR/Cas9, MMEJ, NHEJ, TALEN, genome editing, knock-in, zebrafish
- MeSH Terms
- Animals, Genetically Modified/genetics
- CRISPR-Cas Systems/genetics*
- Clustered Regularly Interspaced Short Palindromic Repeats/genetics*
- Gene Editing/methods*
- Zebrafish Proteins/genetics*
- 28272984 Full text @ Bioengineered
Morita, H., Taimatsu, K., Yanagi, K., Kawahara, A. (2017) Exogenous gene integration mediated by genome editing technologies in zebrafish. Bioengineered. 8(3):287-295.
Genome editing technologies, such as transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/ CRISPR-associated protein (Cas) systems, can induce DNA double-strand breaks (DSBs) at the targeted genomic locus, leading to frameshift-mediated gene disruption in the process of DSB repair. Recently, the technology-induced DSBs followed by DSB repairs are applied to integrate exogenous genes into the targeted genomic locus in various model organisms. In addition to a conventional knock-in technology mediated by homology-directed repair (HDR), novel knock-in technologies using refined donor vectors have also been developed with the genome editing technologies based on other DSB repair mechanisms, including non-homologous end joining (NHEJ) and microhomology-mediated end joining (MMEJ). Therefore, the improved knock-in technologies would contribute to freely modify the genome of model organisms.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes