PUBLICATION
Exposure to 1,2-Propanediol Impacts Early Development of Zebrafish (Danio rerio) and Induces Hyperactivity
- Authors
- Massarsky, A., Abdel, A., Glazer, L., Levin, E.D., Di Giulio, R.T.
- ID
- ZDB-PUB-170308-7
- Date
- 2017
- Source
- Zebrafish 14(3): 216-222 (Journal)
- Registered Authors
- Di Giulio, Richard T.
- Keywords
- 1,2-propanediol (propylene glycol), behavior, early development, electronic cigarettes, zebrafish
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects*
- Disease Models, Animal
- Edema/chemically induced
- Edema/epidemiology
- Embryo, Nonmammalian/drug effects*
- Heart Diseases/chemically induced
- Heart Diseases/epidemiology
- Hyperkinesis/chemically induced
- Hyperkinesis/epidemiology*
- Larva/drug effects
- Larva/growth & development
- Pharmaceutical Vehicles
- Propylene Glycol/toxicity*
- Swimming/physiology
- Teratogens/toxicity
- Yolk Sac/drug effects
- Zebrafish/embryology
- Zebrafish/growth & development*
- PubMed
- 28266909 Full text @ Zebrafish
Citation
Massarsky, A., Abdel, A., Glazer, L., Levin, E.D., Di Giulio, R.T. (2017) Exposure to 1,2-Propanediol Impacts Early Development of Zebrafish (Danio rerio) and Induces Hyperactivity. Zebrafish. 14(3):216-222.
Abstract
The use of electronic cigarettes (e-cigarettes) is increasing as an alternative to tobacco burning cigarettes; however, their safety remains to be fully determined. The long-term effects of e-cigarettes are unknown, including the effects of maternal e-cigarette use on pre- and postnatal development. Additional research on the safety of e-cigarettes is needed. Especially useful would be information from high- and moderate-throughput economic model systems. This study investigates the effects of 1,2-propanediol, which was identified as the main component of e-cigarette liquid, on early development of zebrafish (an in vivo high-throughput model system that was recently proposed for the study of tobacco cigarette and e-cigarette toxicity). Zebrafish embryos were exposed to 1.25% or 2.5% 1,2-propanediol from 6 to 72 h post-fertilization (hpf). We show that exposure to 1,2-propanediol did not significantly affect mortality. Hatching success was significantly lower in 2.5% 1,2-propanediol-exposed embryos at 48 hpf, but at 72 hpf no significant differences were noted. Moreover, exposure to 1,2-propanediol reduced growth and increased the incidence of string heart, pericardial edema, and yolk sac edema. Most importantly, developmental exposure to 1.25% 1,2-propanediol caused hyperactive swimming behavior in larvae. This study demonstrates that 1,2-propanediol has adverse impacts on early development in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping