PUBLICATION

Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells

Authors

Jiang, T., Kindt, K., Wu, D.K.

ID

ZDB-PUB-170308-6

Date

2017

Source

eLIFE 6: (Journal)

Registered Authors

Kindt, Katie

Keywords

chicken, developmental biology, mouse, neuroscience, stem cells, zebrafish

MeSH Terms

Zebrafish/embryology*
Hair Cells, Vestibular/physiology*
Ear, Inner/embryology*
Homeodomain Proteins/metabolism*
Transcription Factors/metabolism*
Organogenesis
Animals
Transcription, Genetic*

(all 8)

PubMed

28266911 Full text @ Elife

Abstract

The asymmetric location of stereociliary bundle (hair bundle) on apical surface of mechanosensory hair cells (HCs) dictates the direction in which a given HC can respond to cues such as sound, head movements, and water pressure. Notably, vestibular sensory organs of the inner ear, the maculae, exhibit a line of polarity reversal (LPR) across which, hair bundles are polarized in a mirror-image pattern. Similarly, HCs in neuromasts of the zebrafish lateral line system are generated as pairs, and two sibling HCs develop opposite hair bundle orientations. Within these sensory organs, expression of the transcription factor Emx2 is restricted to only one side of the LPR in the maculae or one of the two sibling HCs in neuromasts. Emx2 mediates hair bundle polarity reversal in these restricted subsets of HCs and generates the mirror-image pattern of the sensory organs. Downstream effectors of Emx2 control bundle polarity cell-autonomously via heterotrimeric G proteins.

Genes / Markers

Figures

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Expression

Gene	Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
EGFP		emx2^idc5/idc5; vo8Tg	standard conditions	Day 4		lateral crista hair cell	IHC	Fig. 10 S1
EGFP		emx2^idc5/idc5; vo8Tg	standard conditions	Day 4		lateral crista stereocilium bundle	IHC	Fig. 10 S1
EGFP		idc4Tg; vo8Tg	standard conditions	Day 4		lateral crista hair cell	IHC	Fig. 10 S1
EGFP		idc4Tg; vo8Tg	standard conditions	Day 4		lateral crista stereocilium bundle	IHC	Fig. 10 S1
EGFP		idc4Tg; vo8Tg	standard conditions	Day 4		neuromast hair cell stereocilium bundle	IHC	Fig. 10
EGFP		idc4Tg; vo8Tg	standard conditions	Day 4		posterior lateral line neuromast neuromast hair cell	IHC	Fig. 10
EGFP		vo8Tg	standard conditions	Day 4		lateral crista hair cell	IHC	Fig. 10 S1
EGFP		vo8Tg	standard conditions	Day 4		lateral crista stereocilium bundle	IHC	Fig. 10 S1
emx2	Ab1-emx2	emx2^idc5/idc5	standard conditions	Day 4	Not Detected	posterior lateral line neuromast neuromast hair cell	IHC	Fig. 10
emx2	Ab1-emx2	WT	standard conditions	Day 4		posterior lateral line neuromast neuromast hair cell	IHC	Fig. 10

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
emx2^idc5/idc5	standard conditions	Day 4	macula utricle stereocilium bundle orientation macula utricle, abnormal	Fig. 10 S1
emx2^idc5/idc5	standard conditions	Day 4	macula utricle vestibular receptor cell stereocilium organization decreased process quality, abnormal	Fig. 10 S1
emx2^idc5/idc5	standard conditions	Day 4	neuromast hair cell stereocilium bundle orientation neuromast, abnormal	Fig. 10
emx2^idc5/idc5	standard conditions	Day 4	neuromast stereocilium bundle bilateral symmetry, abnormal	Fig. 10
emx2^idc5/idc5	standard conditions	Day 4	neuromast stereocilium bundle unidirectional, abnormal	Fig. 10
emx2^idc5/idc5	standard conditions	Day 4	posterior lateral line neuromast hair cell development decreased process quality, abnormal	Fig. 10
emx2^idc5/idc5	standard conditions	Day 4	posterior lateral line neuromast neuromast hair cell emx2 expression absent, abnormal	Fig. 10
emx2^idc5/idc5	standard conditions	Day 4	posterior lateral line posterior lateral line neuromast development decreased process quality, abnormal	Fig. 10
emx2^idc5/idc5; vo8Tg	standard conditions	Day 4	lateral crista stereocilium bundle orientation lateral crista, abnormal	Fig. 10 S1
emx2^idc5/idc5; vo8Tg	standard conditions	Day 4	lateral crista vestibular receptor cell stereocilium organization decreased process quality, abnormal	Fig. 10 S1

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
idc1Tg	Tg(myo6b:GCaMP6s-CAAX)	Transgenic Insertion
idc4Tg	Tg(myo6b:emx2-2A-NLS-mCherry)	Transgenic Insertion
idc5		Small Deletion	emx2
vo8Tg	Tg(myo6b:actb1-EGFP,myl7:EGFP)	Transgenic Insertion

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
emx2	CRISPR1-emx2	CRISPR
emx2	CRISPR2-emx2	CRISPR

Fish

Fish
emx2^idc5/idc5
emx2^idc5/idc5; vo8Tg
idc1Tg
idc1Tg; idc4Tg
idc4Tg
idc4Tg; vo8Tg
vo8Tg
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-emx2	polyclonal	emx2		Rabbit
Ab1-pvalb	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GCaMP	EFG	GCaMP
mCherry	EFG	mCherry

Mapping

Jiang et al., 2017 ZDB-PUB-170308-6 PMID:28266911

Transcription factor Emx2 controls stereociliary bundle orientation of sensory hair cells

Jiang et al., 2017

ZDB-PUB-170308-6
PMID:28266911