PUBLICATION

Dynamics of miRNA transcriptome during gonadal development of zebrafish

Authors
Presslauer, C., Tilahun Bizuayehu, T., Kopp, M., Fernandes, J.M., Babiak, I.
ID
ZDB-PUB-170307-8
Date
2017
Source
Scientific Reports   7: 43850 (Journal)
Registered Authors
Babiak, Igor, Fernandes, Jorge
Keywords
Germline development, RNA sequencing
MeSH Terms
  • Gonads/embryology
  • Gonads/growth & development
  • Gonads/metabolism*
  • Time Factors
  • Gene Expression Regulation, Developmental*
  • Animals
  • Ovary/embryology
  • Ovary/growth & development
  • Ovary/metabolism
  • High-Throughput Nucleotide Sequencing
  • Female
  • MicroRNAs/genetics*
  • Germ Cells/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Gene Expression Profiling/methods
  • Transcriptome*
  • Male
  • Testis/embryology
  • Testis/growth & development
  • Testis/metabolism
(all 22)
PubMed
28262836 Full text @ Sci. Rep.
Abstract
Studies in non-teleost vertebrates have found microRNAs (miRNAs) to be essential for proper gonadal development. However, comparatively little is known about their role during gonadal development in teleost fishes. So far in zebrafish, a model teleost, transcript profiling throughout gonadal development has not been established because of a tiny size of an organ in juvenile stages and its poor distinguishability from surrounding tissues. We performed small RNA sequencing on isolated gonads of See-Thru-Gonad line, from the undifferentiated state at 3 weeks post fertilization (wpf) to fully mature adults at 24 wpf. We identified 520 gonadal mature miRNAs; 111 of them had significant changes in abundance over time, while 50 miRNAs were either testis- or ovary-enriched significantly in at least one developmental stage. We characterized patterns of miRNA abundance over time including isomiR variants. We identified putative germline versus gonadal somatic miRNAs through differential small RNA sequencing of isolated gametes versus the whole gonads. This report is the most comprehensive analysis of the miRNA repertoire in zebrafish gonads during the sexual development to date and provides an important database from which functional studies can be performed.
Genes / Markers
Figures
Figure Gallery (1 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b18
    		Small Deletion
    w2
      		Point Mutation
      zf45TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        mitfaw2/w2; mpv17b18/b18; zf45Tg
        1 - 1 of 1
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        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
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        Mapping
        No data available
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        Citation
        Presslauer, C., Tilahun Bizuayehu, T., Kopp, M., Fernandes, J.M., Babiak, I. (2017) Dynamics of miRNA transcriptome during gonadal development of zebrafish. Scientific Reports. 7:43850.