PUBLICATION
[Myeloid and erythroid hematopoietic transcription factor expression decline after knockdown of lmna genes in zebrafish embryos].
- Authors
- Hou, S., Wang, Z., Wang, J., He, Z., Shu, L.
- ID
- ZDB-PUB-170302-5
- Date
- 2016
- Source
- Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences 45: 620-625 (Journal)
- Registered Authors
- He, Zhi-Xu, Shu, Li-Ping
- Keywords
- none
- MeSH Terms
-
- Animals
- Down-Regulation/genetics*
- Embryo, Nonmammalian/physiopathology
- GATA1 Transcription Factor/genetics*
- GATA1 Transcription Factor/metabolism
- Gene Knockdown Techniques
- Hematopoiesis
- In Situ Hybridization
- Lamin Type A/genetics
- Lamin Type A/physiology*
- Proto-Oncogene Proteins/genetics*
- Proto-Oncogene Proteins/metabolism
- Trans-Activators/genetics*
- Trans-Activators/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- PubMed
- 28247606 Full text @ Zhejiang Da Xue Xue Bao Yi Xue Ban
Citation
Hou, S., Wang, Z., Wang, J., He, Z., Shu, L. (2016) [Myeloid and erythroid hematopoietic transcription factor expression decline after knockdown of lmna genes in zebrafish embryos].. Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences. 45:620-625.
Abstract
Objective: To investigate the effect of lmna gene down-regulation on early hematopoietic development of zebrafish. Methods: Phosphorodiamidate morpholino oligomer (PMO) technology was used to downregulate lmna gene expression in Zebrafish. Zebrafish embryos injected phosphorodiamidate morpholino antisense oligonucleotide of lmna gene mRNA by microinjection at unicellular stage were taken as the experimental group, and those injected meaningless phosphorodiamidate morpholino antisense oligonucleotide were taken as the control. The embryos were collected at 18, 24, 30 and 36 hpf after the fertilization. The real-time fluorescent quantitative PCR (RT-PCR) and whole embryo in situ hybridization methods were used to detect the expression of myeloid hematopoietic transcription factor pu. 1 and erythroid hematopoietic transcription factor gata1 in zebrafish. Results: RT-PCR showed that the expressions of pu. 1 and gata1 decreased in the experimental group compared with the control group (all P<0.05). Whole embryo in situ hybridization showed that the blue-black positive hybridization signals of pu. 1 and gata1 in experimental group were shallow than those in the control group. Conclusion: Myeloid hematopoietic and erythroid hematopoietic of zebrafish are blocked with the downregulation of lmna gene.
Errata / Notes
[Article in Chinese]
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping