PUBLICATION
FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT
- Authors
- Goto, H., Kimmey, S.C., Row, R.H., Matus, D.Q., Martin, B.L.
- ID
- ZDB-PUB-170301-7
- Date
- 2017
- Source
- Development (Cambridge, England) 144(8): 1412-1424 (Journal)
- Registered Authors
- Martin, Benjamin, Row, Richard H.
- Keywords
- EMT, FGF, Wnt, neuromesodermal progenitor, tailbud, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Epithelial-Mesenchymal Transition*
- Fibroblast Growth Factors/metabolism*
- Gastrula/metabolism
- Imaging, Three-Dimensional
- Mesoderm/cytology
- Mesoderm/embryology*
- Mesoderm/metabolism
- Nervous System/cytology*
- Nervous System/embryology*
- Stem Cells/cytology*
- Stem Cells/metabolism
- T-Box Domain Proteins
- Tail/embryology*
- Vimentin/chemistry
- Vimentin/metabolism
- Wnt Signaling Pathway*
- Xenopus laevis/embryology
- Zebrafish/embryology
- Zebrafish Proteins
- PubMed
- 28242612 Full text @ Development
Citation
Goto, H., Kimmey, S.C., Row, R.H., Matus, D.Q., Martin, B.L. (2017) FGF and canonical Wnt signaling cooperate to induce paraxial mesoderm from tailbud neuromesodermal progenitors through regulation of a two-step EMT. Development (Cambridge, England). 144(8):1412-1424.
Abstract
Mesoderm induction begins during gastrulation. Recent evidence from several vertebrate species indicates that mesoderm induction continues after gastrulation in neuromesodermal progenitors (NMPs) within the posteriormost embryonic structure, the tailbud. It is unclear to what extent the molecular mechanisms of mesoderm induction are conserved between gastrula and post-gastrula stages of development. Fibroblast growth factor (FGF) signaling is required for mesoderm induction during gastrulation through positive transcriptional regulation of the T-box transcription factor brachyury We find in zebrafish that FGF is continuously required for paraxial mesoderm (PM) induction in post-gastrula NMPs. FGF signaling represses the NMP markers brachyury (ntla) and sox2 through regulation of tbx16 and msgn1, thereby committing cells to a PM fate. FGF-mediated PM induction in NMPs functions in tight coordination with canonical Wnt signaling during the epithelial to mesenchymal transition (EMT) from NMP to mesodermal progenitor. Wnt signaling initiates EMT, whereas FGF signaling terminates this event. Our results indicate that germ layer induction in the zebrafish tailbud is not a simple continuation of gastrulation events.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping