PUBLICATION

AMBRA1 links autophagy to cell proliferation and tumorigenesis by promoting c-Myc dephosphorylation and degradation

Authors
Cianfanelli, V., Fuoco, C., Lorente, M., Salazar, M., Quondamatteo, F., Gherardini, P.F., De Zio, D., Nazio, F., Antonioli, M., D'Orazio, M., Skobo, T., Bordi, M., Rohde, M., Dalla Valle, L., Helmer-Citterich, M., Gretzmeier, C., Dengjel, J., Fimia, G.M., Piacentini, M., Di Bartolomeo, S., Velasco, G., Cecconi, F.
ID
ZDB-PUB-170214-261
Date
2015
Source
Nature cell biology   17: 20-30 (Journal)
Registered Authors
Dalla Valle, Luisa, Piacentini, Mauro, Skobo, Tatjana
Keywords
Cell proliferation, Cell signalling, Macroautophagy
MeSH Terms
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/physiology*
  • Animals
  • Autophagy/genetics*
  • Cell Division/genetics
  • Cell Line, Tumor
  • Cell Transformation, Neoplastic/genetics*
  • Female
  • Genes, Tumor Suppressor/physiology*
  • HEK293 Cells
  • Haploinsufficiency*
  • HeLa Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Phosphorylation
  • Protein Phosphatase 2/metabolism
  • Proto-Oncogene Proteins c-myc/metabolism*
  • RNA Interference
  • RNA, Small Interfering
  • TOR Serine-Threonine Kinases/antagonists & inhibitors*
  • Zebrafish
PubMed
25438055 Full text @ Nat. Cell Biol.
Abstract
Inhibition of a main regulator of cell metabolism, the protein kinase mTOR, induces autophagy and inhibits cell proliferation. However, the molecular pathways involved in the cross-talk between these two mTOR-dependent cell processes are largely unknown. Here we show that the scaffold protein AMBRA1, a member of the autophagy signalling network and a downstream target of mTOR, regulates cell proliferation by facilitating the dephosphorylation and degradation of the proto-oncogene c-Myc. We found that AMBRA1 favours the interaction between c-Myc and its phosphatase PP2A and that, when mTOR is inhibited, it enhances PP2A activity on this specific target, thereby reducing the cell division rate. As expected, such a de-regulation of c-Myc correlates with increased tumorigenesis in AMBRA1-defective systems, thus supporting a role for AMBRA1 as a haploinsufficient tumour suppressor gene.
Errata / Notes
This article is corrected by ZDB-PUB-220906-19.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping