PUBLICATION

Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis

Authors
Dyer, C., Blanc, E., Stanley, R.J., Knight, R.D.
ID
ZDB-PUB-170214-236
Date
2015
Source
Neurogenesis (Austin, Tex.)   2: e1057313 (Journal)
Registered Authors
Knight, Robert
Keywords
GSK-3, IWR-1, Tankyrase, axin, mesencephalon, neuron, trigeminal, zebrafish, β-catenin
MeSH Terms
none
PubMed
27606327 Full text @ Neurogenesis (Austin)
Abstract
Interactions between FGF and Wnt/ bcat signaling control development of the midbrain. The nature of this interaction and how these regulate patterning, growth and differentiation is less clear, as it has not been possible to temporally dissect the effects of one pathway relative to the other. We have employed pharmacological and genetic tools to probe the temporal and spatial roles of FGF and Wnt in controlling the specification of early midbrain neurons. We identify a β-catenin (bcat) independent role for GSK-3 in modulating FGF activity and hence neuronal patterning. This function is complicated by an overlap with bcat-dependent regulation of FGF signaling, through the regulation of sprouty4. Additionally we reveal how attenuation of Axin protein function can promote fluctuating levels of bcat activity that are dependent on FGF activity. This highlights the complex nature of the interactions between FGF and Wnt/ bcat and reveals that they act at multiple levels to control each others activity in the midbrain.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cj4TgTransgenic Insertion
    knu3TgTransgenic Insertion
      knu4TgTransgenic Insertion
        ot1TgTransgenic Insertion
          pd1TgTransgenic Insertion
            pt6TgTransgenic Insertion
              tm213
                		Point Mutation
                w25TgTransgenic Insertion
                  w32TgTransgenic Insertion
                    1 - 9 of 9
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                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    No data available
                    Fish
                    No data available
                    Antibodies
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    d2EGFPEFGd2EGFP
                    EGFPEFGEGFP
                    GFPEFGGFP
                    1 - 3 of 3
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                    Mapping
                    No data available
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                    Citation
                    Dyer, C., Blanc, E., Stanley, R.J., Knight, R.D. (2015) Dissecting the role of Wnt signaling and its interactions with FGF signaling during midbrain neurogenesis. Neurogenesis (Austin, Tex.). 2:e1057313.