PUBLICATION

Physical Property Control on the Cellular Uptake Pathway and Spatial Distribution of Nanoparticles in Cells

Authors
Ahn, S., Seo, E., Kim, K.H., Lee, S.J.
ID
ZDB-PUB-170214-120
Date
2015
Source
Journal of biomedical nanotechnology   11: 1051-70 (Journal)
Registered Authors
Keywords
Cancer cell, cellular uptake, gold nanoparticle, two-photon microscopy, x-ray imaging
MeSH Terms
  • Animals
  • Biophysical Phenomena/physiology
  • Cells, Cultured
  • Doxorubicin/pharmacokinetics
  • Embryo, Nonmammalian
  • Endocytosis/physiology*
  • Gold/chemistry
  • Gold/metabolism
  • Gold/pharmacokinetics
  • HeLa Cells
  • Human Umbilical Vein Endothelial Cells/metabolism
  • Humans
  • Hydrogen Bonding
  • Metal Nanoparticles*/chemistry
  • Nanoconjugates/chemistry
  • Signal Transduction
  • Static Electricity
  • Tissue Distribution
  • Zebrafish/embryology
  • Zebrafish/metabolism
PubMed
26353594 Full text @ J Biomed Nanotechnol
Abstract
Nanoparticles have been developed in broad biomedical research in terms of effective cellular interactions to treat and visualize diseased cells. Considering the charge and polar functional groups of proteins that are embedded in cellular membranes, charged nanoparticles have been strategically developed to enhance electrostatic cellular interactions. In this study, we show that cellular uptake efficiency, pathway, and spatial distribution of gold nanoparticles in a cell are significantly modulated based on the surface condition of gold nanoparticles and human cancer cells that were tuned by controlling the pH of the medium and by introducing an electron beam. Cellular uptake efficiency is increased when electrostatic attraction is induced between the cells and the gold nanoparticles. Cell surface modification changes the cellular uptake pathways of the gold nanoparticles and concentrates the gold nanoparticles at the membrane region. Surface modification of the gold nanoparticles also contributes to deep penetration and homogeneous spatial distributions in a cell.
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